Literature summary extracted from
Zhu, H.; Shi, J.; Cregg, J.M.; Woldegiorgis, G.
Reconstitution of highly expressed human heart muscle carnitine palmitoyltransferase I (1997), Biochem. Biophys. Res. Commun., 239, 498-502.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.21 |
overexpression of M-CPT I in Pichia pastoris, containing about 24 copies of the expression vector |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.1.21 |
malonyl-CoA |
CPT II not inhibited; CPT I, native and reconstituted in phospholipid micelles |
Homo sapiens |
|
2.3.1.21 |
Triton X-100 |
recombinant CPT I, complete inactivation at 5%, at least partly reversible |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.3.1.21 |
acyl-CoA + L-carnitine |
Homo sapiens |
- |
CoA + L-acylcarnitine |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.21 |
Homo sapiens |
- |
M-CPT I |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.1.21 |
recombinant M-CPT I from Pichia pastoris |
Homo sapiens |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
2.3.1.21 |
reconstitution of detergent-inactivated recombinant M-CPT I, purified from Pichia pastoris, by removal of detergents in presence of phospholipids |
Homo sapiens |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.3.1.21 |
heart |
M-CPT I |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.21 |
acyl-CoA + L-carnitine |
- |
Homo sapiens |
CoA + L-acylcarnitine |
- |
? |
|