EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.21 | cloning and expression of CPT II in Escherichia coli, overexpression of CPT II in Spodoptera frugiperda Sf 9 cells via baculavirus infection, DNA sequence analysis | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
2.3.1.21 | no loss in activity after 6 freeze/thaw cycles at -70°C or room temeprature, recombinant CPT II | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.21 | 0.034 | - |
palmitoyl-CoA | recombinant CPT II | Rattus norvegicus | |
2.3.1.21 | 0.95 | - |
L-carnitine | recombinant CPT II | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.21 | acyl-CoA + L-carnitine | Rattus norvegicus | - |
CoA + L-acylcarnitine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.21 | Rattus norvegicus | - |
enzyme form CPT II | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.21 | recombinant CPT II from Sf 9 insect cells | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.21 | liver | CPT II | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.21 | 0.43 | - |
recombinant CPT II from Sf 9 insect cells | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.21 | 4°C, pH 7.0-10.0, 24 h stable, recombinant CPT II | Rattus norvegicus |
2.3.1.21 | room temperature, 6.5 h, no loss in activity, recombinant CPT II | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.21 | acyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
2.3.1.21 | palmitoyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-palmitoylcarnitine | - |
? |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.21 | 7 | 10 | stable for 24 h at 4°C, recombinant CPT II | Rattus norvegicus |