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Literature summary extracted from

  • Kamisaka, Y.; Mishra, S.; Nakahara, T.
    Purification and characterization of diacylglycerol acyltransferase from the lipid body fraction of an oleaginous fungus (1997), J. Biochem., 121, 1107-1114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 phosphatidic acid
-
 Umbelopsis ramanniana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 CaCl2 2 mM, inhibits purified enzyme but not activity in lipid body fraction Umbelopsis ramanniana
2.3.1.20 KCl 500 mM, inhibits purified enzyme Umbelopsis ramanniana
2.3.1.20 MgCl2 5 mM, inhibits purified enzyme but not activity in lipid body fraction Umbelopsis ramanniana
2.3.1.20 sodiumdodecyl sulfate irreversible denaturation Umbelopsis ramanniana
2.3.1.20 sphingosine
-
 Umbelopsis ramanniana
2.3.1.20 Trifluoperazine 0.5 mM, in the presence of suboptimal phosphatidic acid concentration Umbelopsis ramanniana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 lipid storage body
-
 Umbelopsis ramanniana
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.20 53000
-
 SDS-PAGE Umbelopsis ramanniana

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Umbelopsis ramanniana
-
 var angulispora, fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 4802fold Umbelopsis ramanniana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.20 3.3
-
-
 Umbelopsis ramanniana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol
-
 Umbelopsis ramanniana CoA + triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,3-dioleoylglycerol
-
 Umbelopsis ramanniana CoA + 2-acyl-1,3-dioleoylglycerol
-
 ?
2.3.1.20 acyl-CoA + lysophosphatidylcholine
-
 Umbelopsis ramanniana CoA + acyllysophosphatidylcholine
-
 ?
2.3.1.20 acyl-CoA + rac-1,2-dioleoylglycerol
-
 Umbelopsis ramanniana CoA + 3-acyl-rac-1,2-dioleoylglycerol
-
 ?
2.3.1.20 acyl-CoA + sn-1,2-diolein
-
 Umbelopsis ramanniana CoA + acyl-sn-1,2-diolein
-
 ?
2.3.1.20 acyl-CoA + sn-2-monoolein
-
 Umbelopsis ramanniana CoA + ?
-
 ?
2.3.1.20 lauroyl-CoA + 1,2-diacylglycerol
-
 Umbelopsis ramanniana CoA + 1,2-diacyl-3-lauroylglycerol
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-diacylglycerol
-
 Umbelopsis ramanniana CoA + 1,2-diacyl-3-oleoylglycerol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.20 40
-
 complete loss of activity after 30 min Umbelopsis ramanniana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7
-
-
 Umbelopsis ramanniana