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Literature summary extracted from

  • Lam, K.S.; Neway, J.O.; Gaucher, G.M.
    In vitro stabilization of 6-methylsalicylic acid synthetase from Penicillium urticae (1988), Can. J. Microbiol., 34, 30-37.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.165 very sensitive to proteolysis, significant stabilization by a combination of NADPH, acetyl-CoA, malonyl-CoA, PMSF, and dithitothreitol Penicillium griseofulvum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.165 NAD+ 0.3 mM, 21% inhibition in crude extracts Penicillium griseofulvum
2.3.1.165 NADH 0.3 mM, 26% inhibition in crude extracts Penicillium griseofulvum
2.3.1.165 NADP+ 0.3 mM, 21% inhibition in crude extracts Penicillium griseofulvum
2.3.1.165 nicotinamide hypoxanthine dinucleotide phosphate reduced form, 0.3 mM, 32% inhibition in crude extracts, oxidized form, 0.3 mM, 24% inhibition in crude extracts Penicillium griseofulvum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH Penicillium griseofulvum first enzyme of patulin biosynthetic pathway 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH Penicillium griseofulvum NRRL 2159A first enzyme of patulin biosynthetic pathway 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.165 Penicillium griseofulvum
-
 NRRL 2159A
-
2.3.1.165 Penicillium griseofulvum
-
 also known as Penicillium urticae
-
2.3.1.165 Penicillium griseofulvum NRRL 2159A
-
 NRRL 2159A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH
-
 Penicillium griseofulvum 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH first enzyme of patulin biosynthetic pathway Penicillium griseofulvum 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH
-
 Penicillium griseofulvum NRRL 2159A 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH first enzyme of patulin biosynthetic pathway Penicillium griseofulvum NRRL 2159A 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.165 30
-
 half-life in crude extracts: 16 min Penicillium griseofulvum
2.3.1.165 30
-
 half-life in crude extracts in the presence of 1 mM PMSF: 53 min Penicillium griseofulvum
2.3.1.165 30
-
 half-life in crude extracts in the presence of 1.5 mM NADPH: 38 min Penicillium griseofulvum
2.3.1.165 30
-
 half-life in crude extracts in the presence of 1.5 mM NADP+: 36 min Penicillium griseofulvum
2.3.1.165 30
-
 half-life in crude extracts in the presence of 1.5 mM NADH: 27 min Penicillium griseofulvum
2.3.1.165 30
-
 half-life in crude extracts in the presence of 1.5 mM NAD+: 27 min Penicillium griseofulvum
2.3.1.165 30
-
 half-life in crude extracts in the presence of 1.5 mM NADPH, 1 mM PMSF, 0.25 mM acetyl-CoA, 0.75 mM malonyl-CoA and 10 mM dithiothreitol: 188 min Penicillium griseofulvum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.165 7.6
-
 more than 60% activity remains after 120 min at 30°C in the presence of PMSF, NADPH, acetyl-CoA and dithiothreitol Penicillium griseofulvum

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.165 NADPH
-
 Penicillium griseofulvum