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Literature summary extracted from

  • Oeljeklaus, S.; Fischer, K.; Gerhardt, B.
    Glyoxysomal acetoacetyl-CoA thiolase and 3-oxoacyl-CoA thiolase from sunflower cotyledons (2002), Planta, 214, 597-607.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.9 labile enzyme, partially stabilized by 1-2 mM dithiothreitol and 20% ethylene glycol Helianthus annuus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.16 palmitoyl-CoA up to 0.03 mM Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.011
-
acetoacetyl-CoA
-
Helianthus annuus
2.3.1.9 0.014
-
CoA
-
Helianthus annuus
2.3.1.16 additional information
-
additional information Km values between 0.003 and 0.007 mM with substrates having a carbon chain length from 6 to 16 carbon atoms, Km values for CoA at high and low acetoacetyl-CoA concentration Helianthus annuus
2.3.1.16 0.027
-
acetoacetyl-CoA
-
Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.9 glyoxysome
-
Helianthus annuus 9514
-
2.3.1.16 glyoxysome
-
Helianthus annuus 9514
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 63000
-
x * 63000, SDS-PAGE Helianthus annuus
2.3.1.9 164000
-
gel filtration Helianthus annuus
2.3.1.16 45000
-
2 * 45000, SDS-PAGE Helianthus annuus
2.3.1.16 75000
-
gel filtration Helianthus annuus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Helianthus annuus
-
acetyl-CoA + acetyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Helianthus annuus
-
sunflower
-
2.3.1.16 Helianthus annuus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 hydroxyapatite, Superdex, Fractogel Helianthus annuus
2.3.1.16
-
Helianthus annuus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 cotyledon
-
Helianthus annuus
-
2.3.1.16 achene
-
Helianthus annuus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 33
-
-
Helianthus annuus
2.3.1.16 352
-
substrate: acetoacetyl-CoA Helianthus annuus
2.3.1.16 1988
-
substrate: 3-oxohexadecanoyl-CoA Helianthus annuus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
Helianthus annuus acetyl-CoA + acetyl-CoA
-
?
2.3.1.9 CoA + acetoacetyl-CoA
-
Helianthus annuus acetyl-CoA + acetyl-CoA
-
ir
2.3.1.16 CoA + 3-oxoacyl-CoA
-
Helianthus annuus acyl-CoA + acetyl-CoA
-
?
2.3.1.16 CoA + acetoacetyl-CoA
-
Helianthus annuus acetyl-CoA + acetyl-CoA
-
?
2.3.1.16 additional information low activity with butyryl-CoA Helianthus annuus ?
-
?
2.3.1.16 additional information activity of the enzyme towards 3-oxoacy-CoAs increased with increasing chain length of the substrate Helianthus annuus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.9 ? x * 63000, SDS-PAGE Helianthus annuus
2.3.1.16 dimer 2 * 45000, SDS-PAGE Helianthus annuus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.16 additional information
-
additional information
-
Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 8.5
-
505 of maximal activity at pH 7.5 and pH 9.5, no activity below pH 6.5 Helianthus annuus
2.3.1.16 8.5
-
pI: 8.0 Helianthus annuus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.16 7.5 9.5 pH 7.5 and 9.5: 50% maximum activity, pH 6.5: no activity Helianthus annuus