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Literature summary extracted from

  • Lukacin.R.; Springob, K.; Urbanke, C.; Ernwein, C.; Schröder, G.; Schröder, J.; Matern, U.
    Native acridone synthases I and II from Ruta graveolens L. form homodimers (1999), FEBS Lett., 448, 135-140.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.159 expression in Escherichia coli strain M15 Ruta graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.159 0.005
-
 malonyl-CoA isozyme II Ruta graveolens
2.3.1.159 0.013
-
 malonyl-CoA isozyme I Ruta graveolens
2.3.1.159 0.062
-
 N-methylanthraniloyl-CoA isozyme I Ruta graveolens
2.3.1.159 0.077
-
 N-methylanthraniloyl-CoA isozyme II Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.159 44000
-
 isozyme I, gel filtration Ruta graveolens
2.3.1.159 45000
-
 about, isozyme II, gel filtration Ruta graveolens
2.3.1.159 81000
-
 isozyme I, analytical ultracentrifugation Ruta graveolens
2.3.1.159 82000
-
 isozyme II, analytical ultracentrifugation Ruta graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens involved in the biosynthesis of all acridone alkaloids CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.159 Ruta graveolens
-
 2 isozymes I and II
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.159 cell suspension culture strain R-20 Ruta graveolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.159 0.18
-
 crude extract Ruta graveolens
2.3.1.159 9.54
-
 purified recombinant isozyme I Ruta graveolens
2.3.1.159 14.16
-
 purified recombinant isozyme II Ruta graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA
-
 Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ?
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA involved in the biosynthesis of all acridone alkaloids Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.159 dimer 2 * 44000-45000, isozyme I and II, analytical ultracentrifugation and SDS-PAGE Ruta graveolens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.159 ACS
-
 Ruta graveolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.159 32
-
 isozyme I Ruta graveolens
2.3.1.159 40
-
 isozyme II Ruta graveolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.159 7
-
 isozyme II Ruta graveolens
2.3.1.159 7.5
-
 isozyme I Ruta graveolens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.159 6 9 isozyme I, assay range Ruta graveolens
2.3.1.159 6 7.5 isozyme II, assay range Ruta graveolens