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Literature summary extracted from

  • Vancura, A.; Haldar, D.
    Purification and characterization of glycerophosphate acyltransferase from rat liver mitochondria (1994), J. Biol. Chem., 269, 27209-27215.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 cardiolipin activation of reconstituted mitochondrial enzyme Rattus norvegicus
2.3.1.15 Lubrol Px above the critical micellar concentration Rattus norvegicus
2.3.1.15 phosphatidylcholine i.e. lecithin, activation of mitochondrial enzyme Rattus norvegicus
2.3.1.15 phosphatidylethanolamine activation of reconstituted mitochondrial acyl transferase Rattus norvegicus
2.3.1.15 phosphatidylglycerol highest activation of reconstituted mitochondrial acyltransferase in the presence of Lubrol PX Rattus norvegicus
2.3.1.15 phosphatidylinositol activation of mitochondrial enzyme Rattus norvegicus
2.3.1.15 phosphatidylserine activation of mitochondrial enzyme Rattus norvegicus
2.3.1.15 Zwittergent 3-14 above the critical micellar concentration Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonic acid trivial name CHAPS, complete inhibition Rattus norvegicus
2.3.1.15 cholate complete inhibition Rattus norvegicus
2.3.1.15 deoxycholate complete inhibition Rattus norvegicus
2.3.1.15 diacylglycerophosphate
-
 Rattus norvegicus
2.3.1.15 Monoacylglycerophosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.0031
-
 palmitoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.006
-
 palmitoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
2.3.1.15 0.0064
-
 stearoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.007
-
 palmitoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.0084
-
 oleoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.011
-
 stearoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
2.3.1.15 0.012
-
 myristoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.013
-
 stearoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.013
-
 oleoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
2.3.1.15 0.015
-
 oleoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.016
-
 myristoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.016
-
 myristoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
2.3.1.15 0.46
-
 glycerol 3-phosphate mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
2.3.1.15 0.82
-
 glycerol 3-phosphate mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
2.3.1.15 0.93
-
 glycerol 3-phosphate mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 mitochondrial outer membrane
-
 Rattus norvegicus 5741
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 85000
-
 1 * 85000, mitochondrial acyltransferase, SDS-PAGE Rattus norvegicus
2.3.1.15 95000 105000 mitochondrial acyltransferase, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 mitochondrial acyltransferase, Bio-Gel HAT, Rotofor, Sephacryl S-300 Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstitution in asolection or phosphatidylglycerol/phosphatidylethanolamine mixture Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.043
-
 mitochondrial acyltransferase reconstituted in asolectin Rattus norvegicus
2.3.1.15 0.19
-
 mitochondrial acyltransferase reconstituted in phosphatidylglycerol/phosphatidylethanolamine mixture Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 myristoyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 monomer 1 * 85000, mitochondrial acyltransferase, SDS-PAGE Rattus norvegicus