BRENDA - Enzyme Database

Acyltransferase systems involved in phospholipid metabolism in Saccharomyces cerevisiae

Yamada, K.; Okuyama, H.; Endo, Y.; Ikezawa, H.; Arch. Biochem. Biophys. 183, 281-289 (1977)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
microsome
-
Saccharomyces cerevisiae
-
-
2.3.1.15
mitochondrion
-
Saccharomyces cerevisiae
5739
-
2.3.1.23
microsome
-
Saccharomyces cerevisiae
-
-
2.3.1.51
membrane
-
Saccharomyces cerevisiae
16020
-
2.3.1.51
microsome
-
Saccharomyces cerevisiae
-
-
2.3.1.51
mitochondrion
the presence of the enzyme in the mitochondrial fraction cannot be explained by the contamination of microsomes in this fraction
Saccharomyces cerevisiae
5739
-
2.3.1.52
membrane
-
Saccharomyces cerevisiae
16020
-
2.3.1.62
microsome
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.62
acyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
Saccharomyces cerevisiae
reaction in phosphatidylcholine metabolism
CoA + phosphatidylcholine
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Saccharomyces cerevisiae
-
OC-2
-
2.3.1.15
Saccharomyces cerevisiae OC-2
-
OC-2
-
2.3.1.23
Saccharomyces cerevisiae
-
OC-2
-
2.3.1.23
Saccharomyces cerevisiae OC-2
-
OC-2
-
2.3.1.51
Saccharomyces cerevisiae
-
OC-2
-
2.3.1.51
Saccharomyces cerevisiae OC-2
-
OC-2
-
2.3.1.52
Saccharomyces cerevisiae
-
OC-2
-
2.3.1.52
Saccharomyces cerevisiae OC-2
-
OC-2
-
2.3.1.62
Saccharomyces cerevisiae
-
OC-2
-
2.3.1.62
Saccharomyces cerevisiae OC-2
-
OC-2
-
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.52
-70°C, membrane preparation, at least 2 months
Saccharomyces cerevisiae
2.3.1.62
-70°C, membrane preparation, at least 2 months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
broad specificity
486375
Saccharomyces cerevisiae
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
broad specificity
486375
Saccharomyces cerevisiae OC-2
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486375
Saccharomyces cerevisiae
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486375
Saccharomyces cerevisiae OC-2
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
r
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486375
Saccharomyces cerevisiae
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
effect of substrate concentration
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486375
Saccharomyces cerevisiae
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
the enzyme utilizes saturated and unsaturated acyl-CoAs at comparable rates
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486375
Saccharomyces cerevisiae
?
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the rate of CoA-liberation is much lower than with 1-acyl-sn-glycerol 3-phosphate
486375
Saccharomyces cerevisiae
CoA + ?
-
486375
Saccharomyces cerevisiae
?
2.3.1.51
additional information
very broad specificity for acyl-CoAs, the enzyme does not seem to discriminate strictly the cis and trans configurations or the position of double bond in the fatty acyl moiety
486375
Saccharomyces cerevisiae
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.52
oleoyl-CoA + 2-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-oleoyl-2-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.52
palmitoyl-CoA + 2-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
acyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + phosphatidylcholine
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
acyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
reaction in phosphatidylcholine metabolism
486375
Saccharomyces cerevisiae
CoA + phosphatidylcholine
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
oleoyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + 1-oleoyl-2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
palmitoyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.52
25
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.23
7
9
-
Saccharomyces cerevisiae
2.3.1.51
7
9
broad
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
microsome
-
Saccharomyces cerevisiae
-
-
2.3.1.15
mitochondrion
-
Saccharomyces cerevisiae
5739
-
2.3.1.23
microsome
-
Saccharomyces cerevisiae
-
-
2.3.1.51
membrane
-
Saccharomyces cerevisiae
16020
-
2.3.1.51
microsome
-
Saccharomyces cerevisiae
-
-
2.3.1.51
mitochondrion
the presence of the enzyme in the mitochondrial fraction cannot be explained by the contamination of microsomes in this fraction
Saccharomyces cerevisiae
5739
-
2.3.1.52
membrane
-
Saccharomyces cerevisiae
16020
-
2.3.1.62
microsome
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.62
acyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
Saccharomyces cerevisiae
reaction in phosphatidylcholine metabolism
CoA + phosphatidylcholine
-
Saccharomyces cerevisiae
?
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.52
-70°C, membrane preparation, at least 2 months
Saccharomyces cerevisiae
2.3.1.62
-70°C, membrane preparation, at least 2 months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
broad specificity
486375
Saccharomyces cerevisiae
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
broad specificity
486375
Saccharomyces cerevisiae OC-2
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486375
Saccharomyces cerevisiae
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486375
Saccharomyces cerevisiae OC-2
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae OC-2
?
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
r
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486375
Saccharomyces cerevisiae
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
effect of substrate concentration
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486375
Saccharomyces cerevisiae
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
the enzyme utilizes saturated and unsaturated acyl-CoAs at comparable rates
486375
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486375
Saccharomyces cerevisiae
?
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the rate of CoA-liberation is much lower than with 1-acyl-sn-glycerol 3-phosphate
486375
Saccharomyces cerevisiae
CoA + ?
-
486375
Saccharomyces cerevisiae
?
2.3.1.51
additional information
very broad specificity for acyl-CoAs, the enzyme does not seem to discriminate strictly the cis and trans configurations or the position of double bond in the fatty acyl moiety
486375
Saccharomyces cerevisiae
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.52
oleoyl-CoA + 2-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-oleoyl-2-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.52
palmitoyl-CoA + 2-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
acyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + phosphatidylcholine
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
acyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
reaction in phosphatidylcholine metabolism
486375
Saccharomyces cerevisiae
CoA + phosphatidylcholine
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
oleoyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + 1-oleoyl-2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
?
2.3.1.62
palmitoyl-CoA + 2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
-
486375
Saccharomyces cerevisiae
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.52
25
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.23
7
9
-
Saccharomyces cerevisiae
2.3.1.51
7
9
broad
Saccharomyces cerevisiae