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Literature summary extracted from

  • Snider, M.D.; Kennedy, E.P.
    Partial purification of glycerophosphate acyltransferase from Escherichia coli (1977), J. Bacteriol., 130, 1072-1083.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 phosphatidylethanolamine seems to be required for optimal activity Escherichia coli
2.3.1.15 Phospholipids absolutely required Escherichia coli
2.3.1.15 Phospholipids bilayers required for activity: phosphatidylethanolamine/-glycerol, ratio 4/1, phosphatidylethanolamine/cardiolipin, ratio 4/1 Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.15 400 mM NaCl or KCl, 5 mM 2-mercaptoethanol, 5 mM MgCl2, stabilize membrane-bound or reconstituted enzyme Escherichia coli
2.3.1.15 glycerol, 20%, stabilizes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 cholate phospholipids restore activity Escherichia coli
2.3.1.15 deoxycholate phospholipids restore activity Escherichia coli
2.3.1.15 Detergents e.g. Brij 35, Brij 58, Lubrol 17A-10, phospholipids restore activity Escherichia coli
2.3.1.15 Triton X-100 phospholipids restore activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.56
-
sn-glycerol 3-phosphate strain 8 Escherichia coli
2.3.1.15 3 3.2 sn-glycerol 3-phosphate strain BB26-36 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 plasma membrane
-
Escherichia coli 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Escherichia coli
-
K-12
-
2.3.1.15 Escherichia coli
-
K-12 derivative strains 8, wild-type genotype
-
2.3.1.15 Escherichia coli
-
A324 and 205
-
2.3.1.15 Escherichia coli
-
overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype
-
2.3.1.15 Escherichia coli 8
-
overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 partial purification Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstitution in phospholipid bilayers Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.105
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate saturated acyl-CoAs preferred Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli 8 CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate saturated acyl-CoAs preferred Escherichia coli 8 CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate 16% of the reaction rate with palmitoyl-CoA Escherichia coli CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate 16% of the reaction rate with palmitoyl-CoA Escherichia coli 8 CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate 4fold better substrate than oleoyl-CoA Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate i.e. n-hexadecanoyl-CoA, best substrate Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate 4fold better substrate than oleoyl-CoA Escherichia coli 8 CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate i.e. n-hexadecanoyl-CoA, best substrate Escherichia coli 8 CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?