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Literature summary extracted from

  • Scheideler, M.A.; Bell, R.M.
    Characterization of active and latent forms of the membrane-associated sn-glycerol-3-phosphate acyltransferase of Escherichia coli (1991), J. Biol. Chem., 266, 14321-14327.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 bovine serum albumin activation at 1 mg/ml Escherichia coli
2.3.1.15 Phospholipids
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 additional information
-
 additional information kinetic cooperativity towards glycerol 3-phosphate and palmitoyl-CoA Escherichia coli
2.3.1.15 0.0046
-
 palmitoyl-CoA strain 8, reconstituted enzyme Escherichia coli
2.3.1.15 0.0056
-
 palmitoyl-CoA strain 8, membrane-associated enzyme Escherichia coli
2.3.1.15 0.006
-
 palmitoyl-CoA strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
2.3.1.15 0.0078
-
 palmitoyl-CoA strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
2.3.1.15 0.012
-
 palmitoyl-CoA strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli
2.3.1.15 0.017
-
 palmitoyl-CoA strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli
2.3.1.15 0.102
-
 sn-glycerol 3-phosphate strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
2.3.1.15 0.105
-
 sn-glycerol 3-phosphate strain 8, membrane-associated enzyme Escherichia coli
2.3.1.15 0.108
-
 sn-glycerol 3-phosphate strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
2.3.1.15 0.79
-
 sn-glycerol 3-phosphate strain 8, reconstituted enzyme Escherichia coli
2.3.1.15 0.92
-
 sn-glycerol 3-phosphate strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli
2.3.1.15 1.1
-
 sn-glycerol 3-phosphate strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 plasma membrane
-
 Escherichia coli 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 additional information
-
 different molecular masses depending on Triton X-100 concentration Escherichia coli
2.3.1.15 88000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Escherichia coli
-
 overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-
2.3.1.15 Escherichia coli
-
 K-12 derivative strains 8, wild-type genotype
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 partial purification Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstitution in cardiolipin vesicles Escherichia coli
2.3.1.15 reconstitution in vesicles Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 3 4 reconstituted enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 ?
-
 Escherichia coli
2.3.1.15 More monomer or oligomer, depending on Triton X-100 concentration Escherichia coli