BRENDA - Enzyme Database

Partial purification and properties of an acyl coenzyme A:sn-glycerol 3-phosphate acyltransferase from rat liver mitochondria

Monroy, G.; Chroboczek Kelker, H.; Pullman, M.E.; J. Biol. Chem. 248, 2845-2852 (1973)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
asolectin
i.e. crude mixture of soybean phospholipids, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
bovine serum albumin
-
Rattus norvegicus
2.3.1.15
phosphatidylcholine
-
Rattus norvegicus
2.3.1.15
phosphatidylserine
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Phospholipids
-
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.3.1.15
asolectin stabilizes cholate-containing preparations
Rattus norvegicus
2.3.1.15
bovine serum albumin stabilizes
Rattus norvegicus
2.3.1.15
cholate inactivates during storage
Rattus norvegicus
2.3.1.15
repeated freezing and thawing inactivates
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
1-palmitoyl-sn-glycerol 3-phosphate
-
Rattus norvegicus
2.3.1.15
cardiolipin
-
Rattus norvegicus
2.3.1.15
cholate
-
Rattus norvegicus
2.3.1.15
diacylglycerophosphate
-
Rattus norvegicus
2.3.1.15
Monoacylglycerophosphate
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
mitochondrial membrane
membrane-bound
Rattus norvegicus
31966
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
Ca2+
activation of microsomal and mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Co2+
-
Rattus norvegicus
2.3.1.15
Mg2+
-
Rattus norvegicus
2.3.1.15
Mn2+
-
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Rattus norvegicus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.3.1.15
lipoprotein
-
Rattus norvegicus
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.15
mitochondrial enzyme, cholate, ammonium sulfate, partial purification
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.15
0.0138
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.15
-70░C, 2 months after the removal of cholate
Rattus norvegicus
2.3.1.15
-70░C, 35% loss of activity after 2 days
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486351
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
no acyl-donors are hexanoyl-CoA, octanoyl-CoA
486351
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
linoleoyl-CoA + sn-glycerol 3-phosphate
2% of activity with palmitoyl-CoA
486351
Rattus norvegicus
CoA + 1-linoleoyl-sn-glycerol 3-phosphate
-
-
-
-
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486351
Rattus norvegicus
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
n-decanoyl-CoA + sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
CoA + 1-n-decanoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
7% of activity with palmitoyl-CoA
486351
Rattus norvegicus
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
poor substrate
486351
Rattus norvegicus
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
asolectin
i.e. crude mixture of soybean phospholipids, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
bovine serum albumin
-
Rattus norvegicus
2.3.1.15
phosphatidylcholine
-
Rattus norvegicus
2.3.1.15
phosphatidylserine
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Phospholipids
-
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.15
asolectin stabilizes cholate-containing preparations
Rattus norvegicus
2.3.1.15
bovine serum albumin stabilizes
Rattus norvegicus
2.3.1.15
cholate inactivates during storage
Rattus norvegicus
2.3.1.15
repeated freezing and thawing inactivates
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
1-palmitoyl-sn-glycerol 3-phosphate
-
Rattus norvegicus
2.3.1.15
cardiolipin
-
Rattus norvegicus
2.3.1.15
cholate
-
Rattus norvegicus
2.3.1.15
diacylglycerophosphate
-
Rattus norvegicus
2.3.1.15
Monoacylglycerophosphate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
mitochondrial membrane
membrane-bound
Rattus norvegicus
31966
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
Ca2+
activation of microsomal and mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Co2+
-
Rattus norvegicus
2.3.1.15
Mg2+
-
Rattus norvegicus
2.3.1.15
Mn2+
-
Rattus norvegicus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.3.1.15
lipoprotein
-
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
mitochondrial enzyme, cholate, ammonium sulfate, partial purification
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.15
0.0138
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.15
-70░C, 2 months after the removal of cholate
Rattus norvegicus
2.3.1.15
-70░C, 35% loss of activity after 2 days
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486351
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
no acyl-donors are hexanoyl-CoA, octanoyl-CoA
486351
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
linoleoyl-CoA + sn-glycerol 3-phosphate
2% of activity with palmitoyl-CoA
486351
Rattus norvegicus
CoA + 1-linoleoyl-sn-glycerol 3-phosphate
-
-
-
-
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486351
Rattus norvegicus
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
n-decanoyl-CoA + sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
CoA + 1-n-decanoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
7% of activity with palmitoyl-CoA
486351
Rattus norvegicus
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
poor substrate
486351
Rattus norvegicus
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486351
Rattus norvegicus
?