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Literature summary extracted from

  • Haldar, D.; Vancura, A.
    Glycerophosphate acyltransferase from liver (1992), Methods Enzymol., 209, 64-72.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 acetone activation of mitochondrial enzyme, inhibition of microsomal enzyme Rattus norvegicus
2.3.1.15 asolectin i.e. crude mixture of soybean phospholipids, activation of mitochondrial enzyme Rattus norvegicus
2.3.1.15 Non-site-specific proteases activation, e.g. proteinase K, subtilisin, not chymotrypsin, trypsin, mitochondrial enzyme, degree of inhibition increases with decreasing ionic strength Rattus norvegicus
2.3.1.15 phosphatidylcholine i.e. lecithin, activation of mitochondrial enzyme Rattus norvegicus
2.3.1.15 phosphatidylethanolamine activation of microsomal enzyme Rattus norvegicus
2.3.1.15 phosphatidylserine activation of mitochondrial enzyme Rattus norvegicus
2.3.1.15 Polymyxin B activation of mitochondrial enzyme, inhibition of microsomal enzyme Rattus norvegicus
2.3.1.15 Proteases activation, microsomal enzyme Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.15 bovine serum albumin stabilizes Rattus norvegicus
2.3.1.15 phospholipids stabilize Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 cardiolipin inhibition of mitochondrial enzyme Rattus norvegicus
2.3.1.15 diacylglycerophosphate i.e. phosphatidic acid, inhibition of mitochondrial enzyme Rattus norvegicus
2.3.1.15 iodoacetamide
-
 Rattus norvegicus
2.3.1.15 Monoacylglycerophosphate i.e. lysophosphatidic acid, inhibition of mitochondrial enzyme Rattus norvegicus
2.3.1.15 additional information liver microsomal enzyme is inhibited by all proteases; liver mitochondrial enzyme, not inhibited by trypsin and chymotrypsin Rattus norvegicus
2.3.1.15 N-ethylmaleimide strong inhibition of microsomal isozyme, mitochondrial enzyme is not inhibited Rattus norvegicus
2.3.1.15 phosphatidylinositol inhibition of microsomal enzyme Rattus norvegicus
2.3.1.15 phosphatidylserine inhibition of microsomal enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.1 0.2 sn-glycerol 3-phosphate microsomal enzyme Rattus norvegicus
2.3.1.15 1
-
 sn-glycerol 3-phosphate mitochondrial enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 microsome
-
 Rattus norvegicus
-
-
2.3.1.15 mitochondrial outer membrane spans transverse plane of outer membrane Rattus norvegicus 5741
-
2.3.1.15 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 Ca2+ activation of microsomal and mitochondrial enzyme Rattus norvegicus
2.3.1.15 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 60000 85000 mitochondrial enzyme, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Rattus norvegicus initial reaction of phosphoglycerol biosynthesis CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 microsomal enzyme, Sepharose 2B, sucrose gradient, partial purification Rattus norvegicus
2.3.1.15 mitochondrial enzyme, Sepharose Q, BioGel HAT, octyl-Sepharose CL-4B, partial purification Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.021
-
 mitochondrial enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate saturated acyl-CoAs preferred Rattus norvegicus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate initial reaction of phosphoglycerol biosynthesis Rattus norvegicus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 monomer 1 * 80000-85000, mitochondrial enzyme, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 6.6 9 both microsomal and mitochondrial enzymes exhibit a broad optimum Rattus norvegicus