BRENDA - Enzyme Database

Glycerophosphate acyltransferase from liver

Haldar, D.; Vancura, A.; Methods Enzymol. 209, 64-72 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
acetone
activation of mitochondrial enzyme, inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
asolectin
i.e. crude mixture of soybean phospholipids, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Non-site-specific proteases
activation, e.g. proteinase K, subtilisin, not chymotrypsin, trypsin, mitochondrial enzyme, degree of inhibition increases with decreasing ionic strength
Rattus norvegicus
2.3.1.15
phosphatidylcholine
i.e. lecithin, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
phosphatidylethanolamine
activation of microsomal enzyme
Rattus norvegicus
2.3.1.15
phosphatidylserine
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Polymyxin B
activation of mitochondrial enzyme, inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
Proteases
activation, microsomal enzyme
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.3.1.15
bovine serum albumin stabilizes
Rattus norvegicus
2.3.1.15
phospholipids stabilize
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
cardiolipin
inhibition of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
diacylglycerophosphate
i.e. phosphatidic acid, inhibition of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
iodoacetamide
-
Rattus norvegicus
2.3.1.15
Monoacylglycerophosphate
i.e. lysophosphatidic acid, inhibition of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
additional information
liver microsomal enzyme is inhibited by all proteases; liver mitochondrial enzyme, not inhibited by trypsin and chymotrypsin
Rattus norvegicus
2.3.1.15
N-ethylmaleimide
strong inhibition of microsomal isozyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
phosphatidylinositol
inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
phosphatidylserine
inhibition of microsomal enzyme
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.1
0.2
sn-glycerol 3-phosphate
microsomal enzyme
Rattus norvegicus
2.3.1.15
1
-
sn-glycerol 3-phosphate
mitochondrial enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
microsome
-
Rattus norvegicus
-
-
2.3.1.15
mitochondrial outer membrane
spans transverse plane of outer membrane
Rattus norvegicus
5741
-
2.3.1.15
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
Ca2+
activation of microsomal and mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Mg2+
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.15
60000
85000
mitochondrial enzyme, gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
Rattus norvegicus
initial reaction of phosphoglycerol biosynthesis
CoA + 1-acyl-sn-glycerol 3-phosphate
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.15
microsomal enzyme, Sepharose 2B, sucrose gradient, partial purification; mitochondrial enzyme, Sepharose Q, BioGel HAT, octyl-Sepharose CL-4B, partial purification
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
0.021
-
mitochondrial enzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486348
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
initial reaction of phosphoglycerol biosynthesis
486348
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.15
monomer
1 * 80000-85000, mitochondrial enzyme, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.15
6.6
9
both microsomal and mitochondrial enzymes exhibit a broad optimum
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
acetone
activation of mitochondrial enzyme, inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
asolectin
i.e. crude mixture of soybean phospholipids, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Non-site-specific proteases
activation, e.g. proteinase K, subtilisin, not chymotrypsin, trypsin, mitochondrial enzyme, degree of inhibition increases with decreasing ionic strength
Rattus norvegicus
2.3.1.15
phosphatidylcholine
i.e. lecithin, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
phosphatidylethanolamine
activation of microsomal enzyme
Rattus norvegicus
2.3.1.15
phosphatidylserine
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Polymyxin B
activation of mitochondrial enzyme, inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
Proteases
activation, microsomal enzyme
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.15
bovine serum albumin stabilizes
Rattus norvegicus
2.3.1.15
phospholipids stabilize
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
cardiolipin
inhibition of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
diacylglycerophosphate
i.e. phosphatidic acid, inhibition of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
iodoacetamide
-
Rattus norvegicus
2.3.1.15
Monoacylglycerophosphate
i.e. lysophosphatidic acid, inhibition of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
additional information
liver microsomal enzyme is inhibited by all proteases; liver mitochondrial enzyme, not inhibited by trypsin and chymotrypsin
Rattus norvegicus
2.3.1.15
N-ethylmaleimide
strong inhibition of microsomal isozyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
phosphatidylinositol
inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
phosphatidylserine
inhibition of microsomal enzyme
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.1
0.2
sn-glycerol 3-phosphate
microsomal enzyme
Rattus norvegicus
2.3.1.15
1
-
sn-glycerol 3-phosphate
mitochondrial enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
microsome
-
Rattus norvegicus
-
-
2.3.1.15
mitochondrial outer membrane
spans transverse plane of outer membrane
Rattus norvegicus
5741
-
2.3.1.15
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
Ca2+
activation of microsomal and mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Mg2+
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.15
60000
85000
mitochondrial enzyme, gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
Rattus norvegicus
initial reaction of phosphoglycerol biosynthesis
CoA + 1-acyl-sn-glycerol 3-phosphate
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
microsomal enzyme, Sepharose 2B, sucrose gradient, partial purification; mitochondrial enzyme, Sepharose Q, BioGel HAT, octyl-Sepharose CL-4B, partial purification
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
0.021
-
mitochondrial enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486348
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
initial reaction of phosphoglycerol biosynthesis
486348
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486348
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.15
monomer
1 * 80000-85000, mitochondrial enzyme, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.15
6.6
9
both microsomal and mitochondrial enzymes exhibit a broad optimum
Rattus norvegicus