Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, Q.; Reinhard, K.; Schiltz, E.; Matern, U.
    Characterization and heterologous expression of hydroxycinnamoyl/benzoyl-CoA:anthranilate N-hydroxycinnamoyl/benzoyltransferase from elicited cell cultures of carnation, Dianthus caryophyllus L (1997), Plant Mol. Biol., 35, 777-789.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.144 expression in Escherichia coli Dianthus caryophyllus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.144 0.009
-
 benzoyl-CoA
-
 Dianthus caryophyllus
2.3.1.144 0.02
-
 anthranilate
-
 Dianthus caryophyllus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.144 44000
-
 gel filtration Dianthus caryophyllus
2.3.1.144 53000
-
 1 * 53000, SDS-PAGE Dianthus caryophyllus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.144 Dianthus caryophyllus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.144 ammonium sulfate, Q-Sepharose, Blue Sepharose, Sephacryl S200, Mono Q Dianthus caryophyllus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.144 0.00000948
-
-
 Dianthus caryophyllus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.144 4-coumaroyl-CoA + anthranilate 127% of activity with benzoyl-CoA Dianthus caryophyllus CoA + N-4-(coumaroyl)anthranilate
-
 ?
2.3.1.144 benzoyl-CoA + anthranilate cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors Dianthus caryophyllus CoA + N-benzoylanthranilate
-
 ?
2.3.1.144 cinnamoyl-CoA + anthranilate 140% of activity with benzoyl-CoA Dianthus caryophyllus CoA + N-cinnamoylanthranilate
-
 ?
2.3.1.144 salicyl-CoA + anthranilate 60% of activity with benzoyl-CoA Dianthus caryophyllus CoA + N-salicylanthranilate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.144 monomer 1 * 53000, SDS-PAGE Dianthus caryophyllus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.144 anthranilate N-hydroxycinammoyl/benzoyltransferase
-
 Dianthus caryophyllus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.144 6.8 7 activity is completely lost if the pH drops below pH 6.5 Dianthus caryophyllus