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Literature summary extracted from

  • Basar, T.; Havlicek, V.; Bezouskova, S.; Hackett, M.; Sebo, P.
    Acylation of lysine 983 is sufficient for toxin activity of Bordetella pertussis adenylate cyclase. Substitutions of alanine 140 modulate acylation site selectivity of the toxin acyltransferase CyaC (2001), J. Biol. Chem., 276, 348-354.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.142 expression of wild-type CyaC acyltransferase and various mutants in Escherichia coli Bordetella pertussis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.142 A140G changed selectivity of acyltransferase for the 2 acetylation sites of adenylate cyclase toxin, a mixture of bi and monoacetylated toxins modified either at both Lys860 and Lys983 or only at Lys860 is generated Bordetella pertussis
2.3.1.142 A140V monoacetylation of adenylate cyclase toxin almost exclusively at Lys983 Bordetella pertussis
2.3.1.142 C67S no effect on adenylate cyclase toxin activation Bordetella pertussis
2.3.1.142 H33D loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
2.3.1.142 H33S loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
2.3.1.142 R141K no effect on adenylate cyclase toxin activation Bordetella pertussis
2.3.1.142 R141L no effect on adenylate cyclase toxin activation Bordetella pertussis
2.3.1.142 S30R loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
2.3.1.142 S30W loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
2.3.1.142 S68T no effect on adenylate cyclase toxin activation Bordetella pertussis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.142 palmitoyl-CoA + adenylate cyclase toxin Bordetella pertussis palmitoylation activates adenylate cyclase toxin CoA + palmitoyladenylate cyclase toxin
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Organism

EC Number Organism UniProt Comment Textmining
2.3.1.142 Bordetella pertussis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.142 palmitoyl-CoA + adenylate cyclase toxin acyltransferase CyaC palmitoylates the conserved lysines 983 and 860 of adenylate cyclase toxin Bordetella pertussis CoA + palmitoyladenylate cyclase toxin
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2.3.1.142 palmitoyl-CoA + adenylate cyclase toxin palmitoylation activates adenylate cyclase toxin Bordetella pertussis CoA + palmitoyladenylate cyclase toxin
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 ?