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Literature summary extracted from

  • Morillas, M.; Gomez-Puertas, P.; Roca, R.; Serra, D.; Asins, G.; Valencia, A.; Hegardt, F.G.
    Structural model of the catalytic core of carnitine palmitoyltransferase I and carnitine octanoyltransferase (COT). Mutation of CPT 1 histidine 473 and alanine 381 and COT alanine 238 impairs the catalytic activity (2001), J. Biol. Chem., 276, 45001-45008.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 expression of CPT I in Saccharomyces cerevisiae Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.21 A381D site-directed mutagenesis, activity is reduced by 86%, Km for acyl-CoA is 6-8fold increased Rattus norvegicus
2.3.1.21 D454G site-directed mutagenesis, loss of activity Rattus norvegicus
2.3.1.21 H473A site-directed mutagenesis, active site mutant, no remaining activity Rattus norvegicus
2.3.1.21 L484P site-directed mutagenesis, loss of activity Rattus norvegicus
2.3.1.21 additional information CPT I mutant with deletion of Arg395 show no activity Rattus norvegicus
2.3.1.21 P479L site-directed mutagenesis, loss of activity Rattus norvegicus
2.3.1.21 R451A site-directed mutagenesis, loss of activity Rattus norvegicus
2.3.1.21 W391A site-directed mutagenesis, loss of activity Rattus norvegicus
2.3.1.21 W452A site-directed mutagenesis, loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA similar inhibition of wild-type CPT I and mutants A381D and H473A Rattus norvegicus
2.3.1.137 malonyl-CoA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 0.0049
-
 palmitoyl-CoA recombinant wild-type CPT I Rattus norvegicus
2.3.1.21 0.033
-
 palmitoyl-CoA mutant A381D Rattus norvegicus
2.3.1.21 0.093
-
 L-carnitine mutant A381D Rattus norvegicus
2.3.1.21 0.129
-
 L-carnitine recombinant wild-type CPT I Rattus norvegicus
2.3.1.137 0.002
-
 acyl-CoA
-
 Rattus norvegicus
2.3.1.137 0.015
-
 octanoyl-CoA
-
 Rattus norvegicus
2.3.1.137 0.0167
-
 acyl-CoA mutant strain expressed in Saccharomyces cerevisiae COT A238D Rattus norvegicus
2.3.1.137 0.172
-
 L-carnitine recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTwt Rattus norvegicus
2.3.1.137 146
-
 L-carnitine recombinant enzyme expressed in Saccharomyces cerevisiae COT238D Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.137 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.1.137 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.21 additional information
-
 amino acid sequence, alignment Rattus norvegicus
2.3.1.21 additional information
-
 3-dimensional structural model of CPT I active site based on X-ray diffraction analysis data from similar rat enoyl-CoA hydratase Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine Rattus norvegicus
-
 CoA + L-acylcarnitine
-
 ?
2.3.1.137 acyl-CoA + L-carnitine Rattus norvegicus beta-oxidation of fatty acids acyl-L-carnitine + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Rattus norvegicus
-
 enzyme form CPT I
-
2.3.1.137 Rattus norvegicus
-
 rat
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.21 palmitoyl-CoA + L-carnitine = CoA + L-palmitoylcarnitine active site three-dimensional structure model Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-acylcarnitine
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-palmitoylcarnitine
-
 ?
2.3.1.137 acyl-CoA + L-carnitine
-
 Rattus norvegicus acyl-L-carnitine + CoA
-
 r
2.3.1.137 acyl-CoA + L-carnitine beta-oxidation of fatty acids Rattus norvegicus acyl-L-carnitine + CoA
-
 r