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Literature summary extracted from
- cDNA cloning, recombinant expression, and site-directed mutagenesis of bovine liver carnitine octanoyltransferase. Arg505 binds the carboxylate group of carnitine (1997), Eur. J. Biochem., 247, 1029-1037.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.1.137 | cDNA cloning by library screening and recombinant expression in Escherichia coli | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.137 | 0.00024 | - |
decanoyl-CoA | mutant [Asn505]COT | Bos taurus |  |
| 2.3.1.137 | 0.00138 | - |
decanoyl-CoA | purified enzyme, wild-type COT | Bos taurus | |
| 2.3.1.137 | 0.0948 | - |
L-carnitine | - |
Bos taurus | |
| 2.3.1.137 | 143 | - |
choline | mutant [Asn505]COT | Bos taurus | |
| 2.3.1.137 | 160 | - |
L-carnitine | mutant [Asn505]COT | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.3.1.137 | mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.137 | 64400 | - |
SDS-PAGE | Bos taurus |
| 2.3.1.137 | 70260 | - |
calculated from cDNA sequence | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.137 | acyl-CoA + L-carnitine | Bos taurus | - |
acyl-L-carnitine + CoA | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.137 | Bos taurus | - |
bovine | - |
| 2.3.1.137 | Bos taurus | - |
beef | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.137 | recombinant enzyme | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.3.1.137 | liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.137 | acyl-CoA + L-carnitine | - |
Bos taurus | acyl-L-carnitine + CoA | - |
r | |
| 2.3.1.137 | choline + L-carnitine | extremely poor substrate | Bos taurus | ? | - |
? | |
| 2.3.1.137 | octanoyl-CoA + L-carnitine | - |
Bos taurus | CoA + L-octanoylcarnitine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.3.1.137 | monomer | 1 * 64400, SDS-PAGE | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.1.137 | COT | - |
Bos taurus |
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Release 2023.1 (January 2023)
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