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Literature summary extracted from

  • Strack, D.; Keller, H.; Weissenböck, G.
    Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves (1987), J. Plant Physiol., 131, 61-73.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.99 EDTA activation Secale cereale
2.3.1.130 DTT activation Secale cereale
2.3.1.131 EDTA activation Secale cereale
2.3.1.132 DTT activates Secale cereale
2.3.1.133 EDTA activation Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 0.05
-
 feruloyl-CoA
-
 Secale cereale
2.3.1.99 4.3
-
 quinate
-
 Secale cereale
2.3.1.130 0.045
-
 feruloyl-CoA
-
 Secale cereale
2.3.1.130 33
-
 galactarate
-
 Secale cereale
2.3.1.131 0.018
-
 sinapoyl-CoA
-
 Secale cereale
2.3.1.131 8
-
 glucarate
-
 Secale cereale
2.3.1.132 0.029
-
 sinapoyl-CoA
-
 Secale cereale
2.3.1.132 10
-
 glucarolactone
-
 Secale cereale
2.3.1.133 0.024
-
 4-coumaroyl-CoA
-
 Secale cereale
2.3.1.133 0.045
-
 caffeoyl-CoA
-
 Secale cereale
2.3.1.133 0.59
-
 shikimate with 4-coumaroyl-CoA Secale cereale
2.3.1.133 83
-
 shikimate with caffeoyl-CoA Secale cereale

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.132 sinapoyl-CoA + glucarolactone Secale cereale best substrate CoA + O-sinapoylglucarolactone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Secale cereale
-
 rye, var. Kustro
-
2.3.1.130 Secale cereale
-
 rye, var. Kustro
-
2.3.1.131 Secale cereale
-
 rye, var. Kustro
-
2.3.1.132 Secale cereale
-
 rye, var. Kustro
-
2.3.1.133 Secale cereale
-
 rye, var. Kustro
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.99 partial Secale cereale
2.3.1.130 partial Secale cereale
2.3.1.131 partial Secale cereale
2.3.1.132 partial Secale cereale
2.3.1.133 partial Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.99 leaf primary Secale cereale
-
2.3.1.130 leaf primary Secale cereale
-
2.3.1.131 leaf primary Secale cereale
-
2.3.1.132 primary leaf
-
 Secale cereale
-
2.3.1.133 leaf primary Secale cereale
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 0.0042
-
-
 Secale cereale
2.3.1.130 0.0033
-
-
 Secale cereale
2.3.1.131 0.01
-
 feruloyl-CoA as donor Secale cereale
2.3.1.132 0.022
-
-
 Secale cereale
2.3.1.133 0.0056
-
-
 Secale cereale

Storage Stability

EC Number Storage Stability Organism
2.3.1.99 -20°C, at least 6 months Secale cereale
2.3.1.130 -20°C, at least 6 months Secale cereale
2.3.1.131 -20°C, at least 6 months Secale cereale
2.3.1.132 -20°C, at least 6 months Secale cereale
2.3.1.133 -20°C, at least 6 months Secale cereale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 4-coumaroyl-CoA + quinate at 39% the rate of the reaction with feruloyl-CoA Secale cereale CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 caffeoyl-CoA + quinate condensation at 10% the rate of the reaction with feruloyl-CoA Secale cereale CoA + 5-O-caffeoylquinate i.e. chlorogenic acid ?
2.3.1.99 feruloyl-CoA + quinate best substrate Secale cereale CoA + O-feruloylquinate
-
 ?
2.3.1.99 p-coumaroyl-CoA + shikimate
-
 Secale cereale CoA + 5-O-(p-coumaroyl)shikimate
-
 ?
2.3.1.99 sinapoyl-CoA + quinate at 21% the rate of the reaction with feruloyl-CoA Secale cereale CoA + 5-O-sinapoylquinate
-
 ?
2.3.1.130 caffeoyl-CoA + galactarate poor substrate Secale cereale CoA + O-caffeoylgalactarate
-
 ?
2.3.1.130 feruloyl-CoA + galactarate best substrate, no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate Secale cereale CoA + O-feruloylgalactarate
-
 ?
2.3.1.130 p-coumaroyl-CoA + galactarate condensation at 69% the rate of the reaction with feruloyl-CoA Secale cereale CoA + O-p-coumaroylgalactarate
-
 ?
2.3.1.130 sinapoyl-CoA + galactarate condensation at 78% the rate of the reaction with feruloyl-CoA Secale cereale CoA + O-sinapoylgalactarate
-
 ?
2.3.1.131 caffeoyl-CoA + glucarate condensation at 8% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + caffeoylglucarate
-
 ?
2.3.1.131 feruloyl-CoA + glucarate condensation at 54% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + feruloylglucarate
-
 ?
2.3.1.131 additional information no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate Secale cereale ?
-
 ?
2.3.1.131 p-coumaroyl-CoA + glucarate condensation at 85% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + p-coumaroylglucarate
-
 ?
2.3.1.131 sinapoyl-CoA + glucarate best substrate Secale cereale CoA + O-sinapoylglucarate
-
 ?
2.3.1.132 caffeoyl-CoA + glucarolactone poor substrate Secale cereale CoA + O-caffeoylglucarolactone
-
 ?
2.3.1.132 feruloyl-CoA + glucarolactone condensation at 37% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + O-feruloylglucarolactone
-
 ?
2.3.1.132 additional information no activity with 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate Secale cereale ?
-
 ?
2.3.1.132 p-coumaroyl-CoA + glucarolactone condensation at 54% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + O-p-coumaroylglucarolactone
-
 ?
2.3.1.132 sinapoyl-CoA + glucarolactone best substrate Secale cereale CoA + O-sinapoylglucarolactone
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate best substrate Secale cereale CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate condensation at 52% the rate of the reaction with p-coumaroyl-CoA Secale cereale CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.133 feruloyl-CoA + shikimate condensation at 22% the rate of the reaction with p-coumaroyl-CoA Secale cereale CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.133 additional information 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate Secale cereale ?
-
 ?
2.3.1.133 sinapoyl-CoA + shikimate condensation at 29% the rate of the reaction with p-coumaroyl-CoA Secale cereale CoA + 5-O-sinapoylshikimate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.130 30
-
 assay at Secale cereale
2.3.1.131 30
-
 assay at Secale cereale

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.99 80
-
 denaturation after 5 min Secale cereale
2.3.1.130 80
-
 denaturation after 5 min Secale cereale
2.3.1.131 80
-
 denaturation after 5 min Secale cereale
2.3.1.132 80
-
 denaturation after 5 min Secale cereale
2.3.1.133 80
-
 denaturation after 5 min Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.99 7.5
-
 100 mM potassium phosphate buffer Secale cereale
2.3.1.130 7.3
-
 25 mM potassium phosphate buffer Secale cereale
2.3.1.131 7.3
-
 100 mM potassium phosphate buffer Secale cereale
2.3.1.132 7.8
-
 50 mM potassium phosphate buffer Secale cereale
2.3.1.133 7.1
-
 200 mM potassium phosphate buffer Secale cereale

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.99 6.9 8 about 80% of maximal activity at pH 6.9 and 8.0 Secale cereale
2.3.1.130 7.1 7.6 about 80% of maximal activity at pH 7.1 and 7.6 Secale cereale
2.3.1.131 6.2 8 about 80% of maximal activity at pH 6.2 and 8.0 Secale cereale
2.3.1.132 7.4 8.2 about 80% of maximal activity at pH 7.4 and pH 8.2 Secale cereale
2.3.1.133 6.3 7.7 about 80% of maximal activity at pH 6.3 and 7.7 Secale cereale