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Literature summary extracted from

  • James, M.O.; Bend, J.R.
    A radiochemical assay for glycine N-acyltransferase activity. Some properties of the enzyme in rat and rabbit (1978), Biochem. J., 172, 285-291.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.13 0.15
-
 phenylacetyl-CoA cosubstrate glycine Oryctolagus cuniculus
2.3.1.13 20
-
 glycine acyl donor phenylacetyl-CoA Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.13 mitochondrion kidney enzyme is located in the matrix, probably not membrane bound Rattus norvegicus 5739
-
2.3.1.13 mitochondrion kidney enzyme is located in the matrix, probably not membrane bound Oryctolagus cuniculus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.13 Oryctolagus cuniculus
-
 New Zealand white rabbits
-
2.3.1.13 Rattus norvegicus
-
 Sprague-Dawley rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.13 kidney
-
 Rattus norvegicus
-
2.3.1.13 kidney
-
 Oryctolagus cuniculus
-
2.3.1.13 liver
-
 Rattus norvegicus
-
2.3.1.13 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.13 0.105
-
 activity in 176000 g supernantant of Triton X-100 solubilized preparations Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.13 acyl-CoA + glycine phenylacetyl-CoA acts as acyl donor Rattus norvegicus CoA + N-acylglycine
-
 ?
2.3.1.13 acyl-CoA + glycine phenylacetyl-CoA acts as acyl donor Oryctolagus cuniculus CoA + N-acylglycine
-
 ?
2.3.1.13 phenylacetyl-CoA + glycine
-
 Oryctolagus cuniculus CoA + N-phenylacetylglycine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.13 37
-
 assay at Rattus norvegicus
2.3.1.13 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.13 8.8 9.5 kidney enzyme Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.13 8 10 approx. 35% of maximal activity at pH 8.0, approx. 40% of maximal activity at pH 10.0, kidney enzyme Oryctolagus cuniculus