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Literature summary extracted from

  • Nandi, D.L.; Lucas, S.V.; Webster, L.T.
    Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization (1979), J. Biol. Chem., 254, 7230-7237.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.13 enzyme fractions from hydroxylapatite column are inactivated by dialysis or concentration by ultracentrifugation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.13 5,5'-dithiobis(2-nitrobenzoate)
-
Bos taurus
2.3.1.13 benzoyl-CoA
-
Bos taurus
2.3.1.13 butyryl-CoA
-
Bos taurus
2.3.1.13 Mg2+
-
Bos taurus
2.3.1.13 Ni2+
-
Bos taurus
2.3.1.13 p-chloromercuribenzoate
-
Bos taurus
2.3.1.13 Zn2+
-
Bos taurus
2.3.1.71 indolacetyl-CoA
-
Bos taurus
2.3.1.71 K+ 200 mM KCl, 44% inhibition Bos taurus
2.3.1.71 Mg2+ 100 mM MgCl2, 90% inhibition Bos taurus
2.3.1.71 Ni2+ 10 mM NiCl2, 98% inhibition Bos taurus
2.3.1.71 phenylacetyl-CoA
-
Bos taurus
2.3.1.71 Zn2+ 1 mM ZnCl2, 13% inhibition Bos taurus
2.3.1.192 5,5'-dithiobis(2-nitrobenzoate) phenylacetyl-CoA partially protects phenylacetyltransferase against 5,5'-dithiobis(2-nitrobenzoate) inactivation Bos taurus
2.3.1.192 acylglycine noncompetitive Bos taurus
2.3.1.192 benzoyl-CoA
-
Bos taurus
2.3.1.192 butyryl-CoA
-
Bos taurus
2.3.1.192 CoA noncompetitve Bos taurus
2.3.1.192 Ni2+ 2 mM, 28% residual activity Bos taurus
2.3.1.192 p-chloromercuribenzoate
-
Bos taurus
2.3.1.192 Zn2+ 1 mM, 46% residual activity Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.71 3
-
glycine reaction with benzoyl-CoA Bos taurus
2.3.1.71 8
-
glycine reaction with salicyl-CoA Bos taurus
2.3.1.71 20
-
benzoyl-CoA
-
Bos taurus
2.3.1.71 45
-
benzoyl-CoA reaction with L-Asn or L-Gln Bos taurus
2.3.1.71 50
-
glycine reaction with butyryl-CoA Bos taurus
2.3.1.71 130
-
L-glutamine
-
Bos taurus
2.3.1.71 130
-
butyryl-CoA
-
Bos taurus
2.3.1.71 170
-
L-asparagine
-
Bos taurus
2.3.1.71 360
-
methylmalonyl-CoA
-
Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.13 mitochondrion
-
Bos taurus 5739
-
2.3.1.71 mitochondrion
-
Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.13 Cs+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
2.3.1.13 K+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
2.3.1.13 Li+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
2.3.1.13 Na+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
2.3.1.13 NH4+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
2.3.1.13 Rb+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
2.3.1.192 additional information addition of monovalent cations is required Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.13 33000
-
SDS-PAGE, sucrose density gradient centrifugation Bos taurus
2.3.1.71 32400
-
sucrose density gradient centrifugation Bos taurus
2.3.1.71 33000
-
1 * 33000, SDS-PAGE Bos taurus
2.3.1.71 33800
-
gel filtration Bos taurus
2.3.1.192 33000
-
gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.13 Bos taurus
-
-
-
2.3.1.71 Bos taurus
-
-
-
2.3.1.192 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.13
-
Bos taurus
2.3.1.71
-
Bos taurus
2.3.1.192
-
Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.13 acyl-CoA + glycine = CoA + N-acylglycine sequential reaction mechanism, acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves and the peptide product dissociates last Bos taurus
2.3.1.192 phenylacetyl-CoA + glycine = CoA + phenylacetylglycine sequential reaction mechanism in which the acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves, and the peptide product dissociates last Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.13 liver
-
Bos taurus
-
2.3.1.71 liver
-
Bos taurus
-
2.3.1.192 liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.71 24
-
-
Bos taurus
2.3.1.192 0.051
-
pH not specified in the publication, temperature not specified in the publication Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.3.1.13 4°C, 1-3 weeks, about 25% loss of activity Bos taurus
2.3.1.71 -70°C, enzyme retains more than half of its activity after several months Bos taurus
2.3.1.71 4°C, 3 weeks, enzyme retains about 80% of its initial activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.13 acyl-CoA + glycine phenylacetyl-CoA or indoleacetyl-CoA can not act as acyl donor Bos taurus CoA + N-acylglycine
-
?
2.3.1.13 acyl-CoA + L-asparagine
-
Bos taurus ?
-
?
2.3.1.13 acyl-CoA + L-glutamine
-
Bos taurus ?
-
?
2.3.1.71 3-methylcrotonyl-CoA + glycine 32% of the activity with benzoyl-CoA Bos taurus CoA + N-3-methylcrotonylglycine
-
?
2.3.1.71 acetyl-CoA + glycine 32% of the activity with benzoyl-CoA Bos taurus CoA + N-acetylglycine
-
?
2.3.1.71 benzoyl-CoA + glycine
-
Bos taurus CoA + N-benzoylglycine
-
?
2.3.1.71 benzoyl-CoA + L-asparagine
-
Bos taurus CoA + N-benzoylasparagine
-
?
2.3.1.71 benzoyl-CoA + L-glutamine weak activity Bos taurus CoA + N-benzoylglutamine
-
?
2.3.1.71 isobutyryl-CoA + glycine 25% of the activity with benzoyl-CoA Bos taurus CoA + N-isobutyrylglycine
-
?
2.3.1.71 isovaleryl-CoA + glycine 103% of the activity with benzoyl-CoA Bos taurus CoA + N-isovalerylglycine
-
?
2.3.1.71 methylmalonyl-CoA + glycine
-
Bos taurus CoA + N-methylmalonylglycine
-
?
2.3.1.71 n-butyryl-CoA + glycine 223% of the activity with benzoyl-CoA Bos taurus CoA + N-butyrylglycine
-
?
2.3.1.71 propionyl-CoA + glycine 53% of the activity with benzoyl-CoA Bos taurus CoA + N-propionylglycine
-
?
2.3.1.71 salicyl-CoA + glycine 35% of the activity with benzoyl-CoA Bos taurus CoA + salicyluric acid
-
?
2.3.1.71 tiglyl-CoA + glycine 115% of the activity with benzoyl-CoA Bos taurus CoA + N-tiglylglycine
-
?
2.3.1.192 indoleacetyl-CoA + glycine
-
Bos taurus N-indoleacetylglycine + CoA
-
?
2.3.1.192 phenylacetyl-CoA + glycine glycine is the preferred acceptor Bos taurus phenylacetylglycine + CoA
-
?
2.3.1.192 phenylacetyl-CoA + L-asparagine stereospecific for the L-isomer of asparagine Bos taurus ? + CoA
-
?
2.3.1.192 phenylacetyl-CoA + L-glutamine stereospecific for the L-isomer of glutamine Bos taurus ? + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.13 monomer 1 * 33000, SDS-PAGE Bos taurus
2.3.1.71 monomer 1 * 33000, SDS-PAGE Bos taurus
2.3.1.192 monomer 1 * 33000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.13 8.4 8.6
-
Bos taurus
2.3.1.71 8.4 8.6
-
Bos taurus
2.3.1.192 8.4 8.6
-
Bos taurus