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Literature summary extracted from

  • Kawasaki, T.; Snyder, F.
    Synthesis of a novel acetylated neutral lipid related to platelet-activating factor by acyl-CoA:1-O-alkyl-2-acetyl-sn-glycerol acyltransferase in HL-60 cells [published erratum appears in J Biol Chem 1988 Jul 25;263(21):10539] (1988), J. Biol. Chem., 263, 2593-2596.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.125 bovine serum albumin activation, molar ratio substrate linoleoyl-CoA/albumin: 2:4:1 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 1-O-Hexadecyl-2-oleoyl-sn-glycerol competitive Homo sapiens
2.3.1.20 DTT above 2 mM Homo sapiens
2.3.1.20 MgCl2 20 mM Homo sapiens
2.3.1.125 1-O-Hexadecyl-2-oleoyl-sn-glycerol competitive inhibitor of 1-hexadecyl-2-acetyl-3-acyl-sn-glycerol synthesis Homo sapiens
2.3.1.125 1-oleoyl-2-acetyl-sn-glycerol mixed-type inhibition Homo sapiens
2.3.1.125 Ca2+ 6% inhibition at 2 mM Homo sapiens
2.3.1.125 Cu2+ 96% inhibition at 2 mM Homo sapiens
2.3.1.125 dithiothreitol
-
 Homo sapiens
2.3.1.125 Mn2+ 35% inhibition at 2 mM Homo sapiens
2.3.1.125 Zn2+ 93% inhibition at 2 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.125 0.0085
-
 linoleoyl-CoA
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Homo sapiens
-
-
2.3.1.125 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+ 1.4fold stimulation Homo sapiens
2.3.1.125 additional information no metal ion requirement Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.125 additional information Homo sapiens related to platelet-activating factor metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Homo sapiens
-
-
-
2.3.1.125 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 HL-60 cell leukemia, HL-60 cell line Homo sapiens
-
2.3.1.125 HL-60 cell
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.20 -20°C, 30 days, microsomal preparation Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol
-
 Homo sapiens CoA + triacylglycerol
-
 ?
2.3.1.20 linoleoyl-CoA + 1,2-diacylglycerol
-
 Homo sapiens CoA + 1,2-diacyl-3-linoleoylglycerol
-
 ?
2.3.1.20 myristoyl-CoA + 1,2-diacylglycerol
-
 Homo sapiens CoA + 1,2-diacyl-3-myristoylglycerol
-
 ?
2.3.1.125 linoleoyl-CoA + 1-O-hexadecyl-2-acetyl-sn-glycerol best substrate, not acetyl-CoA Homo sapiens CoA + 1-O-hexadecyl-2-acetyl-3-linoleoyl-sn-glycerol
-
 ?
2.3.1.125 additional information related to platelet-activating factor metabolism Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 additional information
-
 assay at various temperatures from 24°C to 40°C Homo sapiens
2.3.1.125 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.20 25
-
 labile above, gradual inactivation Homo sapiens
2.3.1.20 40
-
 20% loss of activity after 15 min Homo sapiens
2.3.1.20 50
-
 t1/2: 15 min Homo sapiens
2.3.1.20 65
-
 inactivation after 15 min Homo sapiens
2.3.1.125 45
-
 stable below Homo sapiens
2.3.1.125 58
-
 50% activity after 15 min Homo sapiens
2.3.1.125 65
-
 inactivation Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 6.1
-
 broad Homo sapiens
2.3.1.125 6.8
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.20 0.032
-
 1-O-Hexadecyl-2-oleoyl-sn-glycerol
-
 Homo sapiens
2.3.1.125 0.032
-
 1-O-Hexadecyl-2-oleoyl-sn-glycerol
-
 Homo sapiens