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Literature summary extracted from

  • Mooney, B.P.; Miernyk, J.A.; Randall, D.D.
    Cloning and characterization of the dihydrolipoamide S-acetyltransferase subunit of the plastid pyruvate dehydrogenase complex (E2) from Arabidopsis (1999), Plant Physiol., 120, 443-452.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.12
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.12 chloroplast
-
 Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 50080
-
 calculation from nucleotide sequence Arabidopsis thaliana
2.3.1.12 60000
-
 mature protein, SDS-PAGE Arabidopsis thaliana
2.3.1.12 67000
-
 plastid precursor protein, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Arabidopsis thaliana
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.12 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.12 fusion protein with glutathione S-transferase Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.12 flower
-
 Arabidopsis thaliana
-
2.3.1.12 leaf
-
 Arabidopsis thaliana
-
2.3.1.12 root
-
 Arabidopsis thaliana
-
2.3.1.12 silique
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.12 3.89
-
 fusion protein with glutathione S-transferase Arabidopsis thaliana
2.3.1.12 4.09
-
 after removal of glutathione S-transferase moiety Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Arabidopsis thaliana S-acetyldihydrolipoamide + CoA
-
 ?