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Literature summary extracted from

  • Allen, M.D.; Perham, R.N.
    The catalytic domain of dihydrolipoyl acetyltransferase from the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Expression, purification and reversible denaturation (1997), FEBS Lett., 413, 339-343.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.12 catalytic domain, residues 173-427 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.12 0.013
-
 acetyl-CoA catalytic domain after expression in Escherichia coli Geobacillus stearothermophilus
2.3.1.12 1.2
-
 dihydrolipoamide catalytic domain after expression in Escherichia coli Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 additional information
-
 high molecular mass species in the absence of guanidinium hydrochloride Geobacillus stearothermophilus
2.3.1.12 30000
-
 gel filtration in the presence of guanidinium hydrochloride Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Geobacillus stearothermophilus
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.12 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.12
-
 Geobacillus stearothermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.12 acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine reaction mechanism Geobacillus stearothermophilus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.12 treatment with guanidine hydrochloride results in dissociation into subunits, removal of guanidine hydrochloride results in refolding of the enzyme with 95% of the original activity Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Geobacillus stearothermophilus S-acetyldihydrolipoamide + CoA
-
 ?