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Literature summary extracted from

  • Willms, C.R.; Oliver, R.M.; Henney, H.R.; Mukherjee, B.B.; Reed, L.J.
    alpha-Keto acid dehydrogenase complexes. VI. Dissociation and reconstitution of the dihydrolipoyl transacetylase of Escherichia coli (1967), J. Biol. Chem., 242, 889-897.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 36000
-
 2 * 36000, in the presence of dilute acetic acid Escherichia coli
2.3.1.12 1470000
-
 sedimentation equilibrium experiments in 0.83 M acetic and and 0.005 M sodium chloride Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Escherichia coli
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.12
-
 Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.12 treatment with dilute acetic acid solution results in dissociation into inactive subunits with MW 70000, removal of the acid results in restoration of enzymatic activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Escherichia coli S-acetyldihydrolipoamide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.12 dimer 2 * 36000, in the presence of dilute acetic acid Escherichia coli