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Literature summary extracted from

  • Watanabe, M.; Sofuni, T.; Nohmi, T.
    Involvement of Cys69 residue in the catalytic mechanism of N-hydroxyarylamine O-acetyltransferase of Salmonella typhimurium. Sequence similarity at the amino acid level suggests a common catalytic mechanism of acetyltransferase for S. typhimurium and higher organisms (1992), J. Biol. Chem., 267, 8429-8436.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.118 cloned and expression of wild type and two mutant enzymes in Escherichia coli XL-1-Blue maxi cells, plasmid pYG122 Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.118 A69C reduces enzyme activity without affecting stability and mobility on SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 A69R reduces enzyme activity without affecting stability and mobility on SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.118 0.01
-
 acetyl-CoA reaction with 2-hydroxy-amino-6-methyldipyrido[1,2-alpha: 3',2'-d]imidazole as substrate Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.118 cytosol
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.118 32180
-
 calculated from nucleotide sequence Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium
-
 derived from strain LT2
-
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium
-
 TA 1538
-
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium TA 1538
-
 TA 1538
-
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium TA98
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.118 nucleotide sequence Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.118 0.001
-
 N-acetylation of p-aminobenzoic acid Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 0.517
-
 N-acetylation of 2-aminofluorene Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 1.31
-
 2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole as substrate Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 8.72
-
 N-acetylation of isoniazide Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.118 acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 Salmonella enterica subsp. enterica serovar Typhimurium TA 1538 CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 Salmonella enterica subsp. enterica serovar Typhimurium TA98 CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.118 monomer
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.118 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium