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Literature summary extracted from

  • Saito, K.; Shinohara, A.; Kamataki, T.; Kato, R.
    Metabolic activation of mutagenic N-hydroxyarylamines by O-acetyltransferase in Salmonella typhimurium TA98 (1985), Arch. Biochem. Biophys., 239, 286-295.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.118 1-Nitro-2-naphthol
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 2-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 coenzyme A
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 iodoacetamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 additional information no inhibition by 2,6-dichloro-4-nitrophenol, chloramphenicol, hydroxylamine; no inhibition by paraoxon Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 N-ethylmaleimide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 p-chloromercuribenzoate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 Pentachlorophenol
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 thiolactomycin
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.118 0.0033
-
 acetyl-CoA reaction with O-acetyl transfer to 2-hydroxyamino-6-methyldipyrido[1,2-alpha: 3',2'-d]imidazole as substrate Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.118 48000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.118 acetyl-CoA + an N-hydroxyarylamine Salmonella enterica subsp. enterica serovar Typhimurium involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species CoA + an N-acetoxyarylamine
-
 ?
2.3.1.118 acetyl-CoA + an N-hydroxyarylamine Salmonella enterica subsp. enterica serovar Typhimurium the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates CoA + an N-acetoxyarylamine
-
 ?
2.3.1.118 acetyl-CoA + an N-hydroxyarylamine Salmonella enterica subsp. enterica serovar Typhimurium unknown endogenous substrate in bacteria CoA + an N-acetoxyarylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium TA98
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.118 partial, by ammonium sulfate precipitation, DEAE-cellulose and Sephadex-G150 column chromatography Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 streptomycin, ammonium sulfate precipitation, DEAE Cellulose, Sephadex G-150 chromatograpy Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.118 0.126
-
 2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole as substrate Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.3.1.118 -80°C, at least 3 months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.118 acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole N-hydroxy-2-acetylaminofluorene cannot replace acetyl-CoA Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole N-hydroxy-2-acetylaminofluorene cannot replace acetyl-CoA Salmonella enterica subsp. enterica serovar Typhimurium TA98 CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 acetyl-CoA + 3-hydroxyamino-1-methyl-pyrido[4,3-b]-indole
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetoxy-amino-1-methyl-pyrido[4,3-b]-indole
-
 ?
2.3.1.118 acetyl-CoA + an N-hydroxyarylamine involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species Salmonella enterica subsp. enterica serovar Typhimurium CoA + an N-acetoxyarylamine
-
 ?
2.3.1.118 acetyl-CoA + an N-hydroxyarylamine the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates Salmonella enterica subsp. enterica serovar Typhimurium CoA + an N-acetoxyarylamine
-
 ?
2.3.1.118 acetyl-CoA + an N-hydroxyarylamine unknown endogenous substrate in bacteria Salmonella enterica subsp. enterica serovar Typhimurium CoA + an N-acetoxyarylamine
-
 ?
2.3.1.118 acetyl-CoA + N-hydroxy-2-aminofluorene
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetoxy-2-aminofluorene
-
 ?
2.3.1.118 butyryl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole 11% of the activity compared to acetyl-CoA as acyl donor Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-(butyryloxy)-6-methylpyrido[3',2':4,5]imidazo[1,2-a]pyridin-2-amine
-
 ?
2.3.1.118 butyryl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole 11% of the activity compared to acetyl-CoA as acyl donor Salmonella enterica subsp. enterica serovar Typhimurium TA98 CoA + N-(butyryloxy)-6-methylpyrido[3',2':4,5]imidazo[1,2-a]pyridin-2-amine
-
 ?
2.3.1.118 hexanoyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole 1.3% of the activity compared to acetyl-CoA as acyl donor Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-hexanoyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 malonyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole 19% of the activity compared to acetyl-CoA as acyl donor Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-malonyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 malonyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole 19% of the activity compared to acetyl-CoA as acyl donor Salmonella enterica subsp. enterica serovar Typhimurium TA98 CoA + N-malonyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 additional information the N-acetoxyarylamine products form covalent adducts with cellular macromolecules, e.g. tRNA Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.3.1.118 additional information the N-acetoxyarylamine products form covalent adducts with cellular macromolecules, e.g. tRNA Salmonella enterica subsp. enterica serovar Typhimurium TA98 ?
-
 ?
2.3.1.118 propionyl-CoA + 2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole 89% of the activity compared to acetyl-CoA as acyl donor Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-propionyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
2.3.1.118 propionyl-CoA + 2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole 89% of the activity compared to acetyl-CoA as acyl donor Salmonella enterica subsp. enterica serovar Typhimurium TA98 CoA + N-propionyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?