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Literature summary extracted from

  • Simms, S.A.; Voige, W.H.; Gilvarg, C.
    Purification and characterization of succinyl-CoA: tetrahydrodipicolinate N-succinyltransferase from Escherichia coli (1984), J. Biol. Chem., 259, 2734-2741.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.117 purification has to be carried out at 0-4°C Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.117 CaCl2 1 mM, 3% inhibition Escherichia coli
2.3.1.117 CoCl2 1 mM, 95% inhibition Escherichia coli
2.3.1.117 CuSO4 1 mM, complete loss of activity Escherichia coli
2.3.1.117 MgCl2 1 mM, 20% inhibition Escherichia coli
2.3.1.117 MnCl2 1 mM, 9% inhibition Escherichia coli
2.3.1.117 additional information not inhibited by EDTA and Fe2+ Escherichia coli
2.3.1.117 N-ethylmaleimide 16 mM, 42% inhibition Escherichia coli
2.3.1.117 p-chloromercuriphenylsulfonate 0.16 mM, 97% inhibition Escherichia coli
2.3.1.117 ZnCl2 1 mM, 88% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.117 0.015
-
succinyl-CoA
-
Escherichia coli
2.3.1.117 0.022
-
tetrahydrodipicolinate
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.117 31000
-
2 * 31000, SDS-PAGE Escherichia coli
2.3.1.117 68000
-
gel filtration Escherichia coli
2.3.1.117 72000
-
sucrose density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.117 succinyl-CoA + (2S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylic acid Escherichia coli involved in the biosynthesis of lysine in bacteria, blue-green algae and higher plants CoA + (2S)-2-[(3-carboxypropanoyl)amino]-6-oxoheptanedioic acid
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.117 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.117 MnCl2, ammonium sulfate, DEAE-cellulose, acid treatment, hydroxyapatite, phenyl-Sepharose, chromatofocusing Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.117 36
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.117 4°C, 0.001 M 2-mercaptoethanol, 80% remaining activity after 1 month Escherichia coli
2.3.1.117 4°C, no 2-mercaptoethanol, 4 days, 80% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.117 succinyl-CoA + (2S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylic acid product formation is strongly preferred Escherichia coli CoA + (2S)-2-[(3-carboxypropanoyl)amino]-6-oxoheptanedioic acid
-
r
2.3.1.117 succinyl-CoA + (2S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylic acid involved in the biosynthesis of lysine in bacteria, blue-green algae and higher plants Escherichia coli CoA + (2S)-2-[(3-carboxypropanoyl)amino]-6-oxoheptanedioic acid
-
r
2.3.1.117 succinyl-CoA + tetrahydrodipicolinate
-
Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.117 dimer 2 * 31000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.117 37
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.117 8.2
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.117 7 9 50% of maximal activity at pH 7, 75% of maximal activity at pH 9.0 Escherichia coli