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Literature summary extracted from

  • Negrel, J.; Javelle, F.
    Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures (1997), Eur. J. Biochem., 247, 1127-1135.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.110 ethanol addition of 14% leads to 2fold increase of enzyme activity Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.110 ammonium sulfate 20% activity at 0.5 M Nicotiana tabacum
2.3.1.110 D-tyrosine methyl ester
-
 Nicotiana tabacum
2.3.1.110 DL-tyrosine methyl ester
-
 Nicotiana tabacum
2.3.1.110 L-tyrosine benzyl ester irreversible binding to tyramine binding site Nicotiana tabacum
2.3.1.110 L-tyrosine ethyl ester
-
 Nicotiana tabacum
2.3.1.110 L-tyrosine methyl ester
-
 Nicotiana tabacum
2.3.1.110 L-tyrosine-tert-butyl ester
-
 Nicotiana tabacum
2.3.1.110 L-tyrosineamide
-
 Nicotiana tabacum
2.3.1.110 L-tyrosinol
-
 Nicotiana tabacum
2.3.1.110 N-Methyltyramine
-
 Nicotiana tabacum
2.3.1.110 NaCl 20% activity at 0.5 M Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.110 0.0006
-
 feruloyl-CoA
-
 Nicotiana tabacum
2.3.1.110 0.001
-
 sinapoyl-CoA
-
 Nicotiana tabacum
2.3.1.110 0.002
-
 4-coumaroyl-CoA
-
 Nicotiana tabacum
2.3.1.110 0.002
-
 isoferuloyl-CoA
-
 Nicotiana tabacum
2.3.1.110 0.0043
-
 tyramine
-
 Nicotiana tabacum
2.3.1.110 0.0047
-
 cinnamoyl-CoA
-
 Nicotiana tabacum
2.3.1.110 0.0113
-
 octopamine
-
 Nicotiana tabacum
2.3.1.110 0.17
-
 4-hydroxyphenylpropylamine
-
 Nicotiana tabacum
2.3.1.110 0.202
-
 dopamine
-
 Nicotiana tabacum
2.3.1.110 1.57
-
 noradrenaline
-
 Nicotiana tabacum
2.3.1.110 1.585
-
 3-methoxytyramine
-
 Nicotiana tabacum
2.3.1.110 4.276
-
 beta-phenylethylamine
-
 Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.110 24000
-
 SDS-PAGE Nicotiana tabacum
2.3.1.110 48000
-
 gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.110 feruloyl-CoA + tyramine Nicotiana tabacum
-
 CoA + feruloyltyramine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.110 Nicotiana tabacum
-
 cv. Xanthi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.110 homogeneity Nicotiana tabacum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.110 feruloyl-CoA + tyramine = CoA + N-feruloyltyramine mechanism Nicotiana tabacum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.110 4.2
-
-
 Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.110 4-coumaroyl-CoA + tyramine
-
 Nicotiana tabacum CoA + N-(4-coumaroyl)tyramine
-
 ?
2.3.1.110 caffeoyl-CoA + tyramine
-
 Nicotiana tabacum CoA + N-caffeoyltyramine
-
 ?
2.3.1.110 cinnamoyl-CoA + tyramine
-
 Nicotiana tabacum CoA + cinnamoyltyramine
-
 ?
2.3.1.110 feruloyl-CoA + (4-hydroxyphenyl)propylamine
-
 Nicotiana tabacum CoA + feruloyl-(4-hydroxyphenyl)propylamine
-
 ?
2.3.1.110 feruloyl-CoA + 3-methoxytyramine
-
 Nicotiana tabacum CoA + feruloyl-3-methoxytyramine
-
 ?
2.3.1.110 feruloyl-CoA + beta-phenylethylamine
-
 Nicotiana tabacum CoA + feruloyl-beta-phenylethylamine
-
 ?
2.3.1.110 feruloyl-CoA + dopamine
-
 Nicotiana tabacum CoA + feruloyldopamine
-
 ?
2.3.1.110 feruloyl-CoA + noradrenaline
-
 Nicotiana tabacum CoA + feruloylnoradrenaline
-
 ?
2.3.1.110 feruloyl-CoA + octopamine
-
 Nicotiana tabacum CoA + feruloyloctopamine
-
 ?
2.3.1.110 feruloyl-CoA + tyramine
-
 Nicotiana tabacum CoA + feruloyltyramine
-
 ?
2.3.1.110 feruloyl-CoA + tyramine low specificity for cinnamoyl-CoA derivates and hydroxyphenethylamines Nicotiana tabacum CoA + feruloyltyramine
-
 ?
2.3.1.110 isoferuloyl-CoA + tyramine
-
 Nicotiana tabacum CoA + isoferuloyltyramine
-
 ?
2.3.1.110 sinapoyl-CoA + tyramine
-
 Nicotiana tabacum CoA + sinapoyltyramine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.110 dimer 2 * 24000, SDS-PAGE Nicotiana tabacum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.110 0.003
-
 L-tyrosine benzyl ester
-
 Nicotiana tabacum
2.3.1.110 0.018
-
 L-tyrosine-tert-butyl ester
-
 Nicotiana tabacum
2.3.1.110 0.02
-
 L-tyrosine ethyl ester
-
 Nicotiana tabacum
2.3.1.110 0.026
-
 L-tyrosine methyl ester
-
 Nicotiana tabacum
2.3.1.110 0.046
-
 L-tyrosinol
-
 Nicotiana tabacum
2.3.1.110 0.055
-
 DL-tyrosine methyl ester
-
 Nicotiana tabacum
2.3.1.110 0.58
-
 D-tyrosine methyl ester
-
 Nicotiana tabacum
2.3.1.110 2
-
 L-tyrosineamide
-
 Nicotiana tabacum
2.3.1.110 2.2
-
 N-Methyltyramine
-
 Nicotiana tabacum