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Literature summary extracted from

  • Negrel, J.; Martin, C.
    The biosynthesis of feruloyltyramine in Nicotiana tabacum (1984), Phytochemistry, 23, 2797-2801.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.110 no loss of activity during dialysis for 16 h at 4°C Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.110 0.0062
-
 feruloyl-CoA + tyramine Nicotiana tabacum
2.3.1.110 0.0067
-
 p-Coumaroyl-CoA + tyramine Nicotiana tabacum
2.3.1.110 0.01
-
 cinnamoyl-CoA + tyramine Nicotiana tabacum
2.3.1.110 0.0125
-
 feruloyl-4'-phosphopantetheine
-
 Nicotiana tabacum
2.3.1.110 0.02
-
 tyramine + feruloyl-CoA Nicotiana tabacum
2.3.1.110 0.05
-
 sinapoyl-CoA + tyramine Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.110 soluble
-
 Nicotiana tabacum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.110 additional information MgCl2 has no influence Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.110 45000
-
 gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.110 feruloyl-CoA + tyramine Nicotiana tabacum
-
 CoA + feruloyltyramine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.110 Nicotiana tabacum
-
 infected with tobacco mosaic virus
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.110 O2-sensitive, mercaptoethanol stabilizes, crude extract Nicotiana tabacum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.110 partial Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.110 leaf
-
 Nicotiana tabacum
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.110 4°C, 10% loss of activity within 7 days, concentrated enzyme Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.110 4-coumaroyl-CoA + tyramine
-
 Nicotiana tabacum CoA + N-(4-coumaroyl)tyramine i.e. 4-hydroxycinnamoyltyramine ?
2.3.1.110 cinnamoyl-CoA + tyramine
-
 Nicotiana tabacum CoA + cinnamoyltyramine
-
 ?
2.3.1.110 feruloyl-4'-phosphopantetheine + tyramine
-
 Nicotiana tabacum 4'-phosphopantetheine + feruloyltyramine
-
 ?
2.3.1.110 feruloyl-CoA + 3-methoxytyramine
-
 Nicotiana tabacum CoA + feruloyl-3-methoxytyramine
-
 ?
2.3.1.110 feruloyl-CoA + dopamine
-
 Nicotiana tabacum CoA + feruloyldopamine
-
 ?
2.3.1.110 feruloyl-CoA + homotyramine
-
 Nicotiana tabacum CoA + feruloylhomotyramine
-
 ?
2.3.1.110 feruloyl-CoA + N-methyltyramine
-
 Nicotiana tabacum CoA + N-feruloyl-N-methyltyramine
-
 ?
2.3.1.110 feruloyl-CoA + noradrenaline synonym: norepinephrine Nicotiana tabacum CoA + feruloylnoradrenaline
-
 ?
2.3.1.110 feruloyl-CoA + octopamine
-
 Nicotiana tabacum CoA + feruloyloctopamine
-
 ?
2.3.1.110 feruloyl-CoA + synephrine
-
 Nicotiana tabacum CoA + feruloylsynephrine
-
 ?
2.3.1.110 feruloyl-CoA + tyramine
-
 Nicotiana tabacum CoA + feruloyltyramine
-
 ?
2.3.1.110 feruloyl-CoA + tyramine
-
 Nicotiana tabacum CoA + feruloyltyramine mainly the trans-form is produced, only traces of the cis-form can be found ?
2.3.1.110 additional information enzyme has a wide specificity for phenylethylamines and cinnamoyl-CoA thioesters but differs in the affinity for the substrate, thus indicating different isoenzymes Nicotiana tabacum ?
-
 ?
2.3.1.110 sinapoyl-CoA + tyramine
-
 Nicotiana tabacum CoA + sinapoyltyramine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.110 30
-
 assay at Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.110 7.5
-
 feruloyl-CoA Nicotiana tabacum
2.3.1.110 8
-
 sinapoyl-CoA Nicotiana tabacum
2.3.1.110 8.5
-
 cinnamoyl-CoA Nicotiana tabacum
2.3.1.110 9
-
 p-coumaroyl-CoA Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.110 6.5 9 formation of feruloyltyramine, half-maximal activity at pH 6.5 and pH 9.0 Nicotiana tabacum