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Literature summary extracted from
- Tetrahydromethanopterin-specific enzymes from Methanopyrus kandleri (2001), Methods Enzymol., 331, 317-353.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.98.1 | additional information | relatively high concentrations of lyotropic salt is required for activity, 200fold activation | Methanopyrus kandleri |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.98.1 | gene mtd, DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli strain BL21(DE3) | Methanopyrus kandleri |
| 2.3.1.101 | expression in Escherichia coli | Methanopyrus kandleri |
| 3.5.4.27 | gene mch, DNA and amino acid sequence determination, overexpression in Escherichia coli strain BL21(DE3) | Methanopyrus kandleri |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.5.4.27 | purified recombinant enzyme, X-ray diffraction structure determination and analysis at 2.0 A resolution | Methanopyrus kandleri |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.98.1 | 0.006 | - |
methylenetetrahydromethanopterin | - |
Methanosarcina barkeri |  |
| 1.5.98.1 | 0.013 | - |
oxidized coenzyme F420 | - |
Archaeoglobus fulgidus | |
| 1.5.98.1 | 0.017 | - |
methylenetetrahydromethanopterin | - |
Archaeoglobus fulgidus | |
| 1.5.98.1 | 0.02 | - |
oxidized coenzyme F420 | native enzyme, pH 6.0, 65°C, N2-atmosphere | Methanopyrus kandleri | |
| 1.5.98.1 | 0.025 | - |
oxidized coenzyme F420 | - |
Methanosarcina barkeri | |
| 1.5.98.1 | 0.033 | - |
methylenetetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus | |
| 1.5.98.1 | 0.065 | - |
oxidized coenzyme F420 | - |
Methanothermobacter thermautotrophicus | |
| 1.5.98.1 | 0.08 | - |
methylenetetrahydromethanopterin | native enzyme, pH 6.0, 65°C, N2-atmosphere | Methanopyrus kandleri | |
| 2.3.1.101 | 0.05 | - |
formylmethanofuran | - |
Methanopyrus kandleri | |
| 2.3.1.101 | 0.1 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | |
| 3.5.4.27 | 0.03 | - |
formyl-tetrahydromethanopterin | - |
Methylorubrum extorquens | |
| 3.5.4.27 | 0.04 | - |
formyl-tetrahydromethanopterin | pH 8.0, 65°C | Methanopyrus kandleri | |
| 3.5.4.27 | 0.22 | - |
formyl-tetrahydromethanopterin | - |
Methanothermus fervidus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.101 | salts | stimulation of activity by salt 1000fold | Methanopyrus kandleri | |
| 3.5.4.27 | additional information | lyotrophic salts at high concentrations above 1 M are required for enzyme activity, but not for stability, 200fold activation | Methanopyrus kandleri | |
| 3.5.4.27 | additional information | lyotrophic salts at high concentrations above 1 M are required for enzyme activity, but not for stability, 2fold activation | Methanothermus fervidus | |
| 3.5.4.27 | additional information | lyotrophic salts at high concentrations above 1 M are required for enzyme activity, but not for stability, 4fold activation | Methanosarcina barkeri | |
| 3.5.4.27 | additional information | lyotrophic salts at high concentrations above 1 M are required for enzyme activity, but not for stability, 65fold activation | Methanothermobacter thermautotrophicus | |
| 3.5.4.27 | additional information | lyotrophic salts at high concentrations above 1 M are required for enzyme activity, but not for stability, 7fold activation | Methylorubrum extorquens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.98.1 | 29644 | - |
4 * 32000, approximately, SDS-PAGE, 4 * 29644, sequence calculation | Archaeoglobus fulgidus |
| 1.5.98.1 | 29644 | - |
6 * 36000, approximately, SDS-PAGE, 6 * 29644, sequence calculation | Methanothermobacter thermautotrophicus |
| 1.5.98.1 | 30305 | - |
x * 30305, sequence calculation | Methanocaldococcus jannaschii |
| 1.5.98.1 | 31000 | - |
8 * 31000, approximately, SDS-PAGE | Methanosarcina barkeri |
| 1.5.98.1 | 31383 | - |
8 * 36000, approximately, SDS-PAGE, 8 * 31383, sequence calculation | Methanopyrus kandleri |
| 1.5.98.1 | 32000 | - |
4 * 32000, approximately, SDS-PAGE, 4 * 29644, sequence calculation | Archaeoglobus fulgidus |
| 1.5.98.1 | 36000 | - |
6 * 36000, approximately, SDS-PAGE, 6 * 29644, sequence calculation | Methanothermobacter thermautotrophicus |
| 1.5.98.1 | 36000 | - |
8 * 36000, approximately, SDS-PAGE, 8 * 31383, sequence calculation | Methanopyrus kandleri |
| 2.3.1.101 | 35000 | - |
4 * 35000 | Methanopyrus kandleri |
| 3.5.4.27 | 33000 | - |
2 * 33282, sequence calculation, 2 * 33000, SDS-PAGE | Methylorubrum extorquens |
| 3.5.4.27 | 33282 | - |
2 * 33282, sequence calculation, 2 * 33000, SDS-PAGE | Methylorubrum extorquens |
| 3.5.4.27 | 33972 | - |
3 * 33972, sequence calculation, 3 * 41500, SDS-PAGE | Methanopyrus kandleri |
| 3.5.4.27 | 34246 | - |
2 * 34246, sequence calculation, 2 * 41000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 3.5.4.27 | 34851 | - |
2 * 34851, sequence calculation, 2 * 39000, SDS-PAGE | Methanothermus fervidus |
| 3.5.4.27 | 34889 | - |
2 * 34889, sequence calculation, 2 * 41000, SDS-PAGE | Methanosarcina barkeri |
| 3.5.4.27 | 34899 | - |
x * 34899, sequence calculation | Methanocaldococcus jannaschii |
| 3.5.4.27 | 39000 | - |
2 * 34851, sequence calculation, 2 * 39000, SDS-PAGE | Methanothermus fervidus |
| 3.5.4.27 | 41000 | - |
2 * 34246, sequence calculation, 2 * 41000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 3.5.4.27 | 41000 | - |
2 * 34889, sequence calculation, 2 * 41000, SDS-PAGE | Methanosarcina barkeri |
| 3.5.4.27 | 41500 | - |
3 * 33972, sequence calculation, 3 * 41500, SDS-PAGE | Methanopyrus kandleri |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.98.1 | additional information | Methanosarcina barkeri | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
| 1.5.98.1 | additional information | Methanothermobacter thermautotrophicus | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
| 1.5.98.1 | additional information | Methanocaldococcus jannaschii | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
| 1.5.98.1 | additional information | Archaeoglobus fulgidus | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
| 1.5.98.1 | additional information | Methanopyrus kandleri | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | Methanosarcina barkeri | - |
methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | Methanothermobacter thermautotrophicus | - |
methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | Methanocaldococcus jannaschii | - |
methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | Methanothermus fervidus | - |
methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | Methanopyrus kandleri | - |
methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | Methylorubrum extorquens | - |
methenyl-tetrahydromethanopterin + H2O | - |
r | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 2.3.1.101 | additional information | enzyme completely soluble in 80% ammonium sulfate | Methanopyrus kandleri |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.98.1 | Archaeoglobus fulgidus | - |
- |
- |
| 1.5.98.1 | Methanocaldococcus jannaschii | - |
- |
- |
| 1.5.98.1 | Methanopyrus kandleri | - |
hyperthermophilic archeon, methanogen, gene mtd | - |
| 1.5.98.1 | Methanosarcina barkeri | - |
- |
- |
| 1.5.98.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
| 2.3.1.101 | Methanopyrus kandleri | - |
- |
- |
| 3.5.4.27 | Methanocaldococcus jannaschii | - |
- |
- |
| 3.5.4.27 | Methanopyrus kandleri | - |
gene mch | - |
| 3.5.4.27 | Methanosarcina barkeri | - |
- |
- |
| 3.5.4.27 | Methanothermobacter thermautotrophicus | - |
- |
- |
| 3.5.4.27 | Methanothermus fervidus | - |
- |
- |
| 3.5.4.27 | Methylorubrum extorquens | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.5.98.1 | O2 causes slow inactivation | Methanopyrus kandleri |
| 2.3.1.101 | inactivated slowly under oxic conditions, purification in anaerobic chamber | Methanopyrus kandleri |
| 3.5.4.27 | the enzyme is slowly inactivated under oxic conditions | Methanopyrus kandleri |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.98.1 | native enzyme, multistep procedure involving ammonium sulfate fractionation and several chromatographic steps, recombinant from Escherichia coli strain BL21(DE3) | Methanopyrus kandleri |
| 2.3.1.101 | in a anaerobic chamber | Methanopyrus kandleri |
| 3.5.4.27 | recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and ion exchange chromatography, native enzyme over 600fold from cell supernatant by ammonium sulfate fractionation and two steps of ion exchange chromatography, purification requires anaerobic conditions, the enzyme is completely soluble at 80% saturated ammonium sulfate | Methanopyrus kandleri |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.98.1 | additional information | optimal growth at 37°C | Methanosarcina barkeri | - |
| 1.5.98.1 | additional information | optimal growth at 65°C | Methanothermobacter thermautotrophicus | - |
| 1.5.98.1 | additional information | optimal growth at 83°C | Archaeoglobus fulgidus | - |
| 1.5.98.1 | additional information | optimal growth at 85°C | Methanocaldococcus jannaschii | - |
| 1.5.98.1 | additional information | optimal growth on H2 and CO2 at 98°C | Methanopyrus kandleri | - |
| 3.5.4.27 | additional information | optimal growth temperature is 30°C | Methylorubrum extorquens | - |
| 3.5.4.27 | additional information | optimal growth temperature is 37°C | Methanosarcina barkeri | - |
| 3.5.4.27 | additional information | optimal growth temperature is 65°C | Methanothermobacter thermautotrophicus | - |
| 3.5.4.27 | additional information | optimal growth temperature is 83°C | Methanothermus fervidus | - |
| 3.5.4.27 | additional information | optimal growth temperature is 85°C | Methanocaldococcus jannaschii | - |
| 3.5.4.27 | additional information | optimal growth temperature is 98°C | Methanopyrus kandleri | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.98.1 | additional information | - |
- |
Methanosarcina barkeri |
| 1.5.98.1 | additional information | - |
- |
Methanothermobacter thermautotrophicus |
| 1.5.98.1 | additional information | - |
- |
Archaeoglobus fulgidus |
| 1.5.98.1 | additional information | - |
- |
Methanopyrus kandleri |
| 2.3.1.101 | 800 | - |
- |
Methanopyrus kandleri |
| 3.5.4.27 | additional information | - |
- |
Methanothermus fervidus |
| 3.5.4.27 | additional information | - |
- |
Methylorubrum extorquens |
| 3.5.4.27 | 5000 | - |
above, purified native enzyme | Methanopyrus kandleri |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.5.98.1 | -20°C, purified recombinant enzyme, N2-atmosphere, stable for at least 4 weeks | Methanopyrus kandleri |
| 3.5.4.27 | -20°C, purified native enzyme, 1.32 M KCl, 50 mM Tris-HCl, pH 7.0, under N2 atmosphere, several months without significaant loss of activity | Methanopyrus kandleri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanosarcina barkeri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
| 1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanothermobacter thermautotrophicus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
| 1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanocaldococcus jannaschii | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
| 1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Archaeoglobus fulgidus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
| 1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanopyrus kandleri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
| 1.5.98.1 | additional information | enzyme is involved in CO2 reduction to CH4 | Methanosarcina barkeri | ? | - |
? | |
| 1.5.98.1 | additional information | enzyme is involved in CO2 reduction to CH4 | Methanothermobacter thermautotrophicus | ? | - |
? | |
| 1.5.98.1 | additional information | enzyme is involved in CO2 reduction to CH4 | Methanocaldococcus jannaschii | ? | - |
? | |
| 1.5.98.1 | additional information | enzyme is involved in CO2 reduction to CH4 | Archaeoglobus fulgidus | ? | - |
? | |
| 1.5.98.1 | additional information | enzyme is involved in CO2 reduction to CH4 | Methanopyrus kandleri | ? | - |
? | |
| 2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | - |
Methanosarcina barkeri | methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus | methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | - |
Methanocaldococcus jannaschii | methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | - |
Methanothermus fervidus | methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | - |
Methanopyrus kandleri | methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | - |
Methylorubrum extorquens | methenyl-tetrahydromethanopterin + H2O | - |
r | |
| 3.5.4.27 | formyl-tetrahydromethanopterin | substrate isolated and purified from Methanobacterium thermoautotrophicum, overview | Methanopyrus kandleri | methenyl-tetrahydromethanopterin + H2O | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.98.1 | ? | x * 30305, sequence calculation | Methanocaldococcus jannaschii |
| 1.5.98.1 | hexamer | 6 * 36000, approximately, SDS-PAGE, 6 * 29644, sequence calculation | Methanothermobacter thermautotrophicus |
| 1.5.98.1 | octamer | 8 * 31000, approximately, SDS-PAGE | Methanosarcina barkeri |
| 1.5.98.1 | octamer | 8 * 36000, approximately, SDS-PAGE, 8 * 31383, sequence calculation | Methanopyrus kandleri |
| 1.5.98.1 | tetramer | 4 * 32000, approximately, SDS-PAGE, 4 * 29644, sequence calculation | Archaeoglobus fulgidus |
| 2.3.1.101 | tetramer | 4 * 35000 | Methanopyrus kandleri |
| 3.5.4.27 | ? | x * 34899, sequence calculation | Methanocaldococcus jannaschii |
| 3.5.4.27 | dimer | 2 * 33282, sequence calculation, 2 * 33000, SDS-PAGE | Methylorubrum extorquens |
| 3.5.4.27 | dimer | 2 * 34246, sequence calculation, 2 * 41000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 3.5.4.27 | dimer | 2 * 34851, sequence calculation, 2 * 39000, SDS-PAGE | Methanothermus fervidus |
| 3.5.4.27 | dimer | 2 * 34889, sequence calculation, 2 * 41000, SDS-PAGE | Methanosarcina barkeri |
| 3.5.4.27 | trimer | 3 * 33972, sequence calculation, 3 * 41500, SDS-PAGE | Methanopyrus kandleri |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.98.1 | MTD | - |
Methanosarcina barkeri |
| 1.5.98.1 | MTD | - |
Methanothermobacter thermautotrophicus |
| 1.5.98.1 | MTD | - |
Methanocaldococcus jannaschii |
| 1.5.98.1 | MTD | - |
Archaeoglobus fulgidus |
| 1.5.98.1 | MTD | - |
Methanopyrus kandleri |
| 1.5.98.1 | N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanosarcina barkeri |
| 1.5.98.1 | N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanothermobacter thermautotrophicus |
| 1.5.98.1 | N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanocaldococcus jannaschii |
| 1.5.98.1 | N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Archaeoglobus fulgidus |
| 1.5.98.1 | N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanopyrus kandleri |
| 3.5.4.27 | Mch | - |
Methanosarcina barkeri |
| 3.5.4.27 | Mch | - |
Methanothermobacter thermautotrophicus |
| 3.5.4.27 | Mch | - |
Methanocaldococcus jannaschii |
| 3.5.4.27 | Mch | - |
Methanothermus fervidus |
| 3.5.4.27 | Mch | - |
Methanopyrus kandleri |
| 3.5.4.27 | Mch | - |
Methylorubrum extorquens |
| 3.5.4.27 | N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methanosarcina barkeri |
| 3.5.4.27 | N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methanothermobacter thermautotrophicus |
| 3.5.4.27 | N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methanocaldococcus jannaschii |
| 3.5.4.27 | N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methanothermus fervidus |
| 3.5.4.27 | N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methanopyrus kandleri |
| 3.5.4.27 | N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methylorubrum extorquens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.98.1 | 60 | - |
- |
Methanothermobacter thermautotrophicus |
| 1.5.98.1 | 60 | - |
above | Methanosarcina barkeri |
| 1.5.98.1 | 70 | - |
- |
Archaeoglobus fulgidus |
| 1.5.98.1 | 75 | - |
- |
Methanopyrus kandleri |
| 2.3.1.101 | 90 | - |
- |
Methanopyrus kandleri |
| 3.5.4.27 | additional information | - |
optimal growth temperature of the organism is 30°C | Methylorubrum extorquens |
| 3.5.4.27 | additional information | - |
optimal growth temperature of the organism is 37°C | Methanosarcina barkeri |
| 3.5.4.27 | additional information | - |
optimal growth temperature of the organism is 65°C | Methanothermobacter thermautotrophicus |
| 3.5.4.27 | additional information | - |
optimal growth temperature of the organism is 83°C | Methanothermus fervidus |
| 3.5.4.27 | additional information | - |
optimal growth temperature of the organism is 85°C | Methanocaldococcus jannaschii |
| 3.5.4.27 | additional information | - |
optimal growth temperature of the organism is 98°C | Methanopyrus kandleri |
| 3.5.4.27 | 40 | - |
- |
Methylorubrum extorquens |
| 3.5.4.27 | 50 | - |
- |
Methanosarcina barkeri |
| 3.5.4.27 | 85 | - |
- |
Methanothermus fervidus |
| 3.5.4.27 | 95 | - |
- |
Methanopyrus kandleri |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.98.1 | 90 | - |
30 min, stable up to over 90°C | Methanopyrus kandleri |
| 2.3.1.101 | 90 | - |
stable up to, in presence of salts | Methanopyrus kandleri |
| 3.5.4.27 | 60 | - |
stable up to | Methanosarcina barkeri |
| 3.5.4.27 | 60 | - |
stable up to | Methylorubrum extorquens |
| 3.5.4.27 | 65 | - |
stable up to | Methanothermobacter thermautotrophicus |
| 3.5.4.27 | 90 | - |
stable up to | Methanothermus fervidus |
| 3.5.4.27 | 90 | - |
stable at 90°C and above | Methanopyrus kandleri |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.98.1 | 6 | - |
assay at, substrate methylenetetrahydromethanopterin, forward reaction | Methanopyrus kandleri |
| 1.5.98.1 | 8 | - |
assay at, substrate methenyltetrahydromethanopterin, reverse reaction | Methanopyrus kandleri |
| 3.5.4.27 | 8 | - |
assay at | Methanopyrus kandleri |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.98.1 | coenzyme F420 | dependent on | Methanosarcina barkeri | |
| 1.5.98.1 | coenzyme F420 | dependent on | Methanothermobacter thermautotrophicus | |
| 1.5.98.1 | coenzyme F420 | dependent on | Methanocaldococcus jannaschii | |
| 1.5.98.1 | coenzyme F420 | dependent on | Archaeoglobus fulgidus | |
| 1.5.98.1 | coenzyme F420 | dependent on | Methanopyrus kandleri | |
| 1.5.98.1 | additional information | enzyme contains no prosthetic group | Methanopyrus kandleri | |
| 3.5.4.27 | additional information | the enzyme contains no prothetic group | Methanopyrus kandleri |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.5.98.1 | Methanopyrus kandleri | deduced from amino acid sequence | - |
4.2 |
| 1.5.98.1 | Methanothermobacter thermautotrophicus | deduced from amino acid sequnce | - |
4.9 |
| 1.5.98.1 | Archaeoglobus fulgidus | deduced from amino acid sequnce | - |
4.9 |
| 1.5.98.1 | Methanocaldococcus jannaschii | deduced from amino acid sequnce | - |
6 |
| 3.5.4.27 | Methanopyrus kandleri | amino acid sequence calculation | - |
3.8 |
| 3.5.4.27 | Methanothermobacter thermautotrophicus | amino acid sequence calculation | - |
4.2 |
| 3.5.4.27 | Methanosarcina barkeri | amino acid sequence calculation | - |
4.3 |
| 3.5.4.27 | Methanothermus fervidus | amino acid sequence calculation | - |
4.3 |
| 3.5.4.27 | Methylorubrum extorquens | amino acid sequence calculation | - |
4.8 |
| 3.5.4.27 | Methanocaldococcus jannaschii | amino acid sequence calculation | - |
5.5 |
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Release 2023.1 (January 2023)
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