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Literature summary extracted from

  • Pomper, B.K.; Vorholt, J.A.
    Characterization of the formyltransferase from Methylobacterium extorquens AM1 (2001), Eur. J. Biochem., 268, 4769-4775.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.101 expression in Escherichia coli Methylorubrum extorquens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.101 0.03
-
 5,6,7,8-tetrahydromethanopterin
-
 Methylorubrum extorquens
2.3.1.101 0.05
-
 formylmethanofuran
-
 Methylorubrum extorquens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.101 (NH4)2SO4 1 M increases the activity 20fold Methylorubrum extorquens
2.3.1.101 K2HPO4 1.5 M stimulates 10fold Methylorubrum extorquens
2.3.1.101 additional information not: Fe, Mo, V, Wo Methylorubrum extorquens
2.3.1.101 NaCl 1 M increases the activity 20fold Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.101 310000
-
 enzyme complex with three other polypeptides that shows sequence identities with the subunits of formylmethanofuran-dehydrogenase and are required for stability of enzyme, the subunits are present in 2:2:2:2 stoichiometry Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin Methylorubrum extorquens
-
 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.101 Methylorubrum extorquens
-
 gene ffsA
-
2.3.1.101 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 gene ffsA
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.101 if a single chromatographic step was performed 90% loss of activity, in 24 h 100% loss of activity Methylorubrum extorquens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.101 purification of enzyme complex Methylorubrum extorquens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin pathway via enzyme complex Methylorubrum extorquens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.101 additional information
-
-
 Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methylorubrum extorquens methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.101 ? enzyme is the 32000 Da subunit of enzyme complex Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.101 7
-
-
 Methylorubrum extorquens