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Literature summary extracted from

  • Kunow, J.; Shima, S.; Vorholt, J.A.; Thauer, R.K.
    Primary structure and properties of the formyltransferase from the mesophilic Methanosarcina barkeri. Comparison with the enzymes from thermophilic and hyperthermophilic methanogens (1996), Arch. Microbiol., 165, 97-105.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.101 32000
-
 1 * 32000 SDS-PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin Methanosarcina barkeri
-
 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin Methanosarcina barkeri Fusaro / DSM 804
-
 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.101 Methanosarcina barkeri P55301
-
-
2.3.1.101 Methanosarcina barkeri Fusaro / DSM 804 P55301
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.101 recombinant enzyme Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.101 additional information
-
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri Fusaro / DSM 804 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.101 monomer 1 * 32000 SDS-PAGE Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.101 65
-
-
 Methanosarcina barkeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.101 70
-
 up to, in presence of 1.5 M K2HPO4, hyperthermophilic enzyme Methanosarcina barkeri