EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.101 | expression in Escherichia coli | Methanopyrus kandleri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.101 | 0.04 | - |
formylmethanofuran | recombinant enzyme | Methanopyrus kandleri | |
2.3.1.101 | 0.05 | - |
formylmethanofuran | wild-type enzyme | Methanopyrus kandleri | |
2.3.1.101 | 0.1 | - |
5,6,7,8-tetrahydromethanopterin | wild-type enzyme | Methanopyrus kandleri | |
2.3.1.101 | 0.107 | - |
5,6,7,8-tetrahydromethanopterin | recombinant enzyme | Methanopyrus kandleri |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.101 | salts | optimal salt concentration for activity: 1.5-2.0 M | Methanopyrus kandleri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.101 | 35000 | - |
1 * 35000, SDS-PAGE | Methanopyrus kandleri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.101 | Methanopyrus kandleri | Q49610 | strain DSM 6324 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.101 | recombinant enzyme | Methanopyrus kandleri |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.101 | additional information | - |
- |
Methanopyrus kandleri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.101 | monomer | 1 * 35000, SDS-PAGE | Methanopyrus kandleri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.101 | 90 | - |
recombinant and wild-type enzyme | Methanopyrus kandleri |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.101 | 90 | - |
stable up to, in presence of salts | Methanopyrus kandleri |