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Literature summary extracted from

  • Breitung, J.; Thauer, R.K.
    Formylmethanofuran: tetrahydromethanopterin formyltransferase from Methanosarcina barkeri. Identification of N5-formyltetrahydromethanopterin as the product (1990), FEBS Lett., 275, 226-230.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.101 0.04
-
 formylmethanofuran
-
 Methanosarcina barkeri
2.3.1.101 0.04
-
 5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri
2.3.1.101 0.05
-
 formylmethanofuran
-
 Methanothermobacter thermautotrophicus
2.3.1.101 0.06
-
 formylmethanofuran
-
 Methanosarcina barkeri
2.3.1.101 0.06
-
 formylmethanofuran
-
 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.101 32000
-
 gel filtration Methanosarcina barkeri
2.3.1.101 32000
-
 1 * 32000 SDS-PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin Methanosarcina barkeri involved in the formation of methane from CO2 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin Methanothermobacter thermautotrophicus involved in the formation of methane from CO2 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.101 Methanosarcina barkeri
-
-
-
2.3.1.101 Methanothermobacter thermautotrophicus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.101 stable in presence of molecular oxygen Methanosarcina barkeri

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.101
-
 Methanosarcina barkeri
2.3.1.101
-
 Methanothermobacter thermautotrophicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin ternary complex type mechanism Methanosarcina barkeri
a
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin ternary complex type mechanism Methanothermobacter thermautotrophicus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.101 610
-
-
 Methanosarcina barkeri

Storage Stability

EC Number Storage Stability Organism
2.3.1.101 4°C, 50 mM Tricine/KOH, pH 8, stable for 1 week Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methanothermobacter thermautotrophicus methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin involved in the formation of methane from CO2 Methanosarcina barkeri methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin involved in the formation of methane from CO2 Methanothermobacter thermautotrophicus methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.101 monomer 1 * 32000 SDS-PAGE Methanosarcina barkeri
2.3.1.101 monomer 1 * 32000 SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.101 65
-
-
 Methanosarcina barkeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.101 65
-
 stable for more than 4 h Methanosarcina barkeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.101 1970
-
 5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri
2.3.1.101 2770
-
 5,6,7,8-tetrahydromethanopterin
-
 Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.101 7
-
-
 Methanosarcina barkeri