EC Number | General Stability | Organism |
---|---|---|
1.5.98.1 | thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 | Archaeoglobus fulgidus |
2.3.1.101 | salts protect against heat inactivation, order of efficiency: K2HPO4, (NH4)2SO4, KCl, NH4Cl, Na2SO4, Na2HPO4 | Archaeoglobus fulgidus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.98.1 | (NH4)2SO4 | 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus | |
1.5.98.1 | K2HPO4 | 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.98.1 | 0.013 | - |
coenzyme F420 | - |
Archaeoglobus fulgidus | |
1.5.98.1 | 0.017 | - |
5,10-methylenetetrahydromethanopterin | - |
Archaeoglobus fulgidus | |
2.3.1.101 | 0.017 | - |
5,6,7,8-tetrahydromethanopterin | - |
Archaeoglobus fulgidus | |
2.3.1.101 | 0.032 | - |
formylmethanofuran | - |
Archaeoglobus fulgidus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.98.1 | (NH4)2SO4 | 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus | |
1.5.98.1 | K+ | 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus | |
2.3.1.101 | (NH4)2SO4 | can substitute for K2HPO4 in stimulation | Archaeoglobus fulgidus | |
2.3.1.101 | K2HPO4 | 1.5 M stimulates 3.8fold | Archaeoglobus fulgidus | |
2.3.1.101 | K2HPO4 | no activity in absence, assay mixture contains 2 M | Archaeoglobus fulgidus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 32000 | - |
x * 32000, SDS-PAGE | Archaeoglobus fulgidus |
1.5.98.1 | 140000 | - |
gel filtration | Archaeoglobus fulgidus |
2.3.1.101 | 30000 | - |
4 * 30000 SDS-PAGE | Archaeoglobus fulgidus |
2.3.1.101 | 125000 | - |
PAGE | Archaeoglobus fulgidus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.3.1.101 | additional information | enzyme soluble in 60% ammonium sulfate | Archaeoglobus fulgidus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.98.1 | Archaeoglobus fulgidus | - |
- |
- |
2.3.1.101 | Archaeoglobus fulgidus | - |
- |
- |
2.3.1.101 | Archaeoglobus fulgidus VC-19 / DSM 4304 | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.3.1.101 | relatively insensitive towards inactivation by molecular oxygen | Archaeoglobus fulgidus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.98.1 | - |
Archaeoglobus fulgidus |
2.3.1.101 | - |
Archaeoglobus fulgidus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | ternary complex type mechanism | Archaeoglobus fulgidus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 1560 | - |
- |
Archaeoglobus fulgidus |
2.3.1.101 | 1415 | - |
- |
Archaeoglobus fulgidus |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.98.1 | -20°C, stable for several weeks | Archaeoglobus fulgidus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | r | Archaeoglobus fulgidus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | r | Archaeoglobus fulgidus VC-19 / DSM 4304 | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Archaeoglobus fulgidus | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Archaeoglobus fulgidus VC-19 / DSM 4304 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.98.1 | ? | x * 32000, SDS-PAGE | Archaeoglobus fulgidus |
2.3.1.101 | tetramer | 4 * 30000 SDS-PAGE | Archaeoglobus fulgidus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 70 | - |
- |
Archaeoglobus fulgidus |
2.3.1.101 | 70 | - |
- |
Archaeoglobus fulgidus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 90 | - |
thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 | Archaeoglobus fulgidus |
2.3.1.101 | 80 | - |
complete thermostability in presence of 0.5 M K2HPO4 | Archaeoglobus fulgidus |
2.3.1.101 | 90 | - |
stable up to, in presence of salts | Archaeoglobus fulgidus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 5.5 | - |
- |
Archaeoglobus fulgidus |
2.3.1.101 | 5 | - |
- |
Archaeoglobus fulgidus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.98.1 | coenzyme F420 | required | Archaeoglobus fulgidus |