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Literature summary extracted from

  • Uchiyama, C.; Mori, M.; Tatibana, M.
    Subcellular localization and properties of N-acetylglutamate synthase in rat small intestinal mucosa (1981), J. Biochem., 89, 1777-1786.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.1 arginine 1 mM, 2-6fold activation in mitochondria, 1.6fold activation of partially purified enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.1 0.8
-
 acetyl-CoA
-
 Rattus norvegicus
2.3.1.1 2.5
-
 L-glutamate
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.1 acetyl-CoA + L-glutamate Rattus norvegicus enzyme activates carbamoyl-phosphate synthase ammonia in small intestine mucosa, to allow citrulline synthesis in the tissue CoA + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.1 ammonium sulfate, hydroxyapatite Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.1 small intestine mucosa Rattus norvegicus
-
2.3.1.1 small intestine little activity in muscle layer and serosa Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.1 additional information
-
 0.00000017 mmol N-acetylglutamate/min/g mucosa Rattus norvegicus
2.3.1.1 31.6
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.1 acetyl-CoA + glycine
-
 Rattus norvegicus CoA + acetylaminoacetate
-
 ?
2.3.1.1 acetyl-CoA + L-aspartate 3.0% of activity with L-glutamate Rattus norvegicus CoA + N-acetyl-L-aspartate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate enzyme activates carbamoyl-phosphate synthase ammonia in small intestine mucosa, to allow citrulline synthesis in the tissue Rattus norvegicus CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 propionyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-propionyl-L-glutamate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.1 60
-
 5 min, complete loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.1 8.5
-
-
 Rattus norvegicus