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Literature summary extracted from

  • Yokota, A.; Sasajima, K.
    Enzymatic formation of a new monosaccharide, 1-deoxy-D-altro-heptulose phosphate, from DL-acetoin and D-ribose 5-phosphate by a transketolase mutant of Bacillus pumilus (1983), Agric. Biol. Chem., 47, 1545-1553.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.2.1.4 thiamine diphosphate, or Mg2+, or 2-mercaptoethanol stabilizes Bacillus pumilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.4 cytoplasm
-
Bacillus pumilus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.4 Mg2+ requirement Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.4 120000
-
gel filtration Bacillus pumilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.4 3-hydroxybutan-2-one + D-ribose 5-phosphate Bacillus pumilus i.e. acetoin acetaldehyde + 1-deoxy-D-altro-heptulose 7-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.4 Bacillus pumilus
-
transketolase mutant strain BG2532
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.4 partial Bacillus pumilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.4 0.0145
-
-
Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.4 3-hydroxybutan-2-one + D-ribose 5-phosphate i.e. acetoin Bacillus pumilus acetaldehyde + 1-deoxy-D-altro-heptulose 7-phosphate
-
?
2.2.1.4 methylacetoin + D-ribose 5-phosphate
-
Bacillus pumilus acetone + 1-deoxy-D-altro-heptulose 7-phosphate
-
?
2.2.1.4 additional information no substrates are acetaldehyde, pyruvate, acetone, hydroxyacetone, acetylacetone i.e. 2,4-pentanedione, propanetriol, diacetyl i.e. 2,3-butanedione, 2,3-butanediol, 1,2,3-butanetriol, 2-butanone Bacillus pumilus ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.4 37
-
assay at Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.4 37
-
stable for 1 h at pH 6.0-8.0 in the presence of cofactors and 2-mercaptoethanol Bacillus pumilus
2.2.1.4 80
-
inactivation after 3 min Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.4 8.5 9
-
Bacillus pumilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.2.1.4 6 8 stable for 1 h at 37°C in the presence of cofactors and 2-mercaptoethanol Bacillus pumilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.4 thiamine diphosphate requirement, thiamine diphosphate protein Bacillus pumilus