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Literature summary extracted from

  • O'Connor, M.L.; Quayle, J.R.
    Pentose phosphate-dependent fixation of formaldehyde by methanol-grown Hansenula polymorpha and Candida boidinii (1980), J. Gen. Microbiol., 120, 219-225.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.2.1.3 glycerol, 20% w/v, stabilizes during purification [Candida] boidinii
2.2.1.3 glycerol, 20% w/v, stabilizes during purification Ogataea angusta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde [Candida] boidinii
-
 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde Ogataea angusta
-
 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde Ogataea angusta DL-1
-
 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.3 Ogataea angusta
-
 methanol-grown
-
2.2.1.3 Ogataea angusta DL-1
-
 methanol-grown
-
2.2.1.3 [Candida] boidinii
-
 methanol grown
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.3 partial [Candida] boidinii
2.2.1.3 partial Ogataea angusta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.3 0.285
-
-
 [Candida] boidinii

Storage Stability

EC Number Storage Stability Organism
2.2.1.3 4°C, partially purified, inactivation within 12 h [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 Ogataea angusta glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 Ogataea angusta DL-1 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 fructose 6-phosphate + formaldehyde
-
 [Candida] boidinii dihydroxyacetone + erythrose 4-phosphate
-
 ?
2.2.1.3 fructose 6-phosphate + formaldehyde
-
 Ogataea angusta dihydroxyacetone + erythrose 4-phosphate
-
 ?
2.2.1.3 fructose 6-phosphate + formaldehyde
-
 Ogataea angusta DL-1 dihydroxyacetone + erythrose 4-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.3 7.4 7.6 markedly less activity in Tris than in phosphate or glycylglycine buffer [Candida] boidinii
2.2.1.3 7.4 7.6 markedly less activity in Tris than in phosphate or glycylglycine buffer Ogataea angusta