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Literature summary extracted from

  • Kato, N.; Higuchi, T.; Sakazawa, C.; Nishizawa, T.; Tani, Y.; Yamada, H.
    Purification and properties of a transketolase responsible for formaldehyde fixation in a methanol-utilizing yeast, candida boidinii (Kloeckera sp.) No. 2201 (1982), Biochim. Biophys. Acta, 715, 143-150.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.2.1.3 Mg2+, 5 mM, thiamine diphosphate 0.5 mM, DTT, 1 mM, EDTA 1 mM stabilize [Candida] boidinii
2.2.1.3 PMSF, 0.024% w/v, stabilizes [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.3 1,10-phenanthroline
-
 [Candida] boidinii
2.2.1.3 As2O3
-
 [Candida] boidinii
2.2.1.3 Co2+
-
 [Candida] boidinii
2.2.1.3 CuSO4
-
 [Candida] boidinii
2.2.1.3 EDTA
-
 [Candida] boidinii
2.2.1.3 FeCl3
-
 [Candida] boidinii
2.2.1.3 HgCl2
-
 [Candida] boidinii
2.2.1.3 iodoacetate
-
 [Candida] boidinii
2.2.1.3 PCMB
-
 [Candida] boidinii
2.2.1.3 Zn2+
-
 [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.3 0.42
-
 glyceraldehyde 3-phosphate
-
 [Candida] boidinii
2.2.1.3 0.43
-
 formaldehyde
-
 [Candida] boidinii
2.2.1.3 0.52
-
 dihydroxyacetone
-
 [Candida] boidinii
2.2.1.3 1
-
 D-xylulose 5-phosphate
-
 [Candida] boidinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.3 Mg2+ requirement [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.3 55000
-
 alpha4, 4 * 55000, SDS-PAGE [Candida] boidinii
2.2.1.3 190000
-
 gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde [Candida] boidinii pathway for formaldehyde fixation glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.3 [Candida] boidinii
-
 methanol grown
-
2.2.1.3 [Candida] boidinii
-
 Kloeckera sp. No. 2201
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.3
-
 [Candida] boidinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.3 3.88
-
-
 [Candida] boidinii

Storage Stability

EC Number Storage Stability Organism
2.2.1.3 5°C, at least 1 month in buffer with stabilizing agents [Candida] boidinii
2.2.1.3 5°C, inactivation within 10 days with one of the stabilizing agents of the buffer omitted [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone r
2.2.1.3 D-xylulose 5-phosphate + formaldehyde pathway for formaldehyde fixation [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone r
2.2.1.3 hydroxypyruvate + formaldehyde efficiency as glycolaldehyde donor [Candida] boidinii CO2 + dihydroxyacetone
-
 ?
2.2.1.3 additional information with xylulose 5-phosphate as glycolyl donor: aliphatic aldehydes (C1-C7), glycolaldehyde and glyceraldehyde are better acceptors than ribose 5-phosphate [Candida] boidinii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.3 tetramer alpha4, 4 * 55000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.3 35
-
-
 [Candida] boidinii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.3 35 45 maximal activity at 35°C and about 80% of maximal activity at 45°C [Candida] boidinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.3 40
-
 10 min stable [Candida] boidinii
2.2.1.3 50
-
 inactivation after 10 min [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.3 7
-
-
 [Candida] boidinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.3 6.5 7.5 about 80% of maximal activity at pH 6.5 and 7.5 [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.3 thiamine diphosphate
-
 [Candida] boidinii