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Literature summary extracted from

  • Demuynck, C.; Fisson, F.; Bennani-Baiti, I.; Samaki, H.; Mani, J.C.
    Immunoaffinity purification of transketolases from yeast and spnich leaves (1990), Agric. Biol. Chem., 54, 3073-3078.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 Rabbit antibodies polyclonal Saccharomyces cerevisiae
2.2.1.1 Rabbit antibodies not Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+ requirement Saccharomyces cerevisiae
2.2.1.1 Mg2+ requirement Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 74200
-
 alpha2, 2 * 74200, SDS-PAGE Saccharomyces cerevisiae
2.2.1.1 100000
-
 1 * 100000, SDS-PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Saccharomyces cerevisiae pathway in non-oxidative sequence of pentose cycle sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Spinacia oleracea pathway in non-oxidative sequence of pentose cycle sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Saccharomyces cerevisiae
-
 baker's yeast
-
2.2.1.1 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 immunoaffinity chromatography Saccharomyces cerevisiae
2.2.1.1 immunoaffinity chromatography Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 18
-
-
 Saccharomyces cerevisiae
2.2.1.1 40
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate pathway in non-oxidative sequence of pentose cycle Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate pathway in non-oxidative sequence of pentose cycle Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.1 hydroxypyruvate + D-glyceraldehyde 3-phosphate enantioselective Saccharomyces cerevisiae CO2 + ribulose 5-phosphate
-
 ?
2.2.1.1 hydroxypyruvate + D-glyceraldehyde 3-phosphate enantioselective Spinacia oleracea CO2 + ribulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.1 dimer
-
 Spinacia oleracea
2.2.1.1 dimer alpha2, 2 * 74200, SDS-PAGE Saccharomyces cerevisiae
2.2.1.1 monomer 1 * 100000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 7.5
-
-
 Saccharomyces cerevisiae
2.2.1.1 8
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate i.e. functional form of vitamin B1 Saccharomyces cerevisiae
2.2.1.1 thiamine diphosphate i.e. functional form of vitamin B1 Spinacia oleracea
2.2.1.1 thiamine diphosphate 2 catalytic sites per enzyme molecule Saccharomyces cerevisiae
2.2.1.1 thiamine diphosphate 1 catalytic site per enzyme molecule Spinacia oleracea