Any feedback?
Literature summary extracted from
- Purification and properties of transketolase from Candida utilis (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 1325-1337.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.2.1.1 | Mg2+ or thiamine diphosphate stabilizes | Cyberlindnera jadinii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.2.1.1 | arsenate | - |
Cyberlindnera jadinii |  |
| 2.2.1.1 | phosphate | - |
Cyberlindnera jadinii | |
| 2.2.1.1 | sulfate | - |
Cyberlindnera jadinii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.2.1.1 | 0.0054 | - |
thiamine diphosphate | - |
Cyberlindnera jadinii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.2.1.1 | Mg2+ | requirement | Cyberlindnera jadinii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | 78000 | - |
alpha2, 2 * 78000, SDS-PAGE | Cyberlindnera jadinii |
| 2.2.1.1 | 163000 | - |
gel filtration | Cyberlindnera jadinii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.2.1.1 | D-xylulose 5-phosphate + D-ribose 5-phosphate | Cyberlindnera jadinii | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.2.1.1 | Cyberlindnera jadinii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.2.1.1 | no isozymes, inseparable from triosephosphate isomerase EC 5.3.1.1 | Cyberlindnera jadinii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | 25 | - |
- |
Cyberlindnera jadinii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.2.1.1 | D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Cyberlindnera jadinii | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.2.1.1 | dimer | alpha2, 2 * 78000, SDS-PAGE | Cyberlindnera jadinii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | 37 | - |
assay at | Cyberlindnera jadinii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | additional information | - |
pI: 4.8 | Cyberlindnera jadinii |
| 2.2.1.1 | 7.8 | - |
- |
Cyberlindnera jadinii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | 5.8 | 9.5 | about half-maximal activity at pH 5.8 and 9.5 | Cyberlindnera jadinii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | additional information | - |
thiamine diphosphate enhances pH-stability | Cyberlindnera jadinii |
| 2.2.1.1 | 5 | - |
denaturation below | Cyberlindnera jadinii |
| 2.2.1.1 | 6 | 10 | stable in the presence of cofactors | Cyberlindnera jadinii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.2.1.1 | thiamine diphosphate | - |
Cyberlindnera jadinii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
