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Literature summary extracted from

  • Gross, M.D.; Eggen, M.A.; Simon, A.M.; Van Pilsum, J.F.
    The purification and charcterization of human kidney L-arginine:glycine amidinotransferase (1986), Arch. Biochem. Biophys., 251, 747-755.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.4.1 medicine presence of the enzyme in serum or urine may prove useful in development of kidney disease diagnosis, hyperornithinemia, an autosomal recessive disease caused by decreased enzyme activity, enzyme activity is also dimished in thyrotoxicosis and myotonic muscular dystrophy Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.4.1 2.3
-
 arginine
-
 Sus scrofa
2.1.4.1 2.4
-
 arginine beta-transamidinase Rattus norvegicus
2.1.4.1 2.5
-
 glycine
-
 Homo sapiens
2.1.4.1 2.5
-
 arginine
-
 Homo sapiens
2.1.4.1 2.8
-
 arginine alpha-transamidinase Rattus norvegicus
2.1.4.1 3
-
 glycine alpha-transamidinase Rattus norvegicus
2.1.4.1 3.1
-
 glycine beta-transamidinase Rattus norvegicus
2.1.4.1 18
-
 glycine
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.4.1 42000
-
 2 * 42000, alpha-transamidinase, SDS-PAGE, 2 * 44000, beta-transamidinase, SDS-PAGE Rattus norvegicus
2.1.4.1 44000
-
 2 * 44000, SDS-PAGE Homo sapiens
2.1.4.1 44000
-
 2 * 42000, alpha-transamidinase, SDS-PAGE, 2 * 44000, beta-transamidinase, SDS-PAGE Rattus norvegicus
2.1.4.1 82600
-
 alpha-transamidinase, sedimentation equilibrium Rattus norvegicus
2.1.4.1 83300
-
 beta-transamidinase, sedimentation equilibrium Rattus norvegicus
2.1.4.1 89000
-
 gel filtration, equilibrium sedimentation Homo sapiens
2.1.4.1 100000
-
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.4.1 L-arginine + glycine Homo sapiens
-
 L-ornithine + guanidinoacetate
-
 r
2.1.4.1 L-arginine + glycine Rattus norvegicus
-
 L-ornithine + guanidinoacetate
-
 r
2.1.4.1 L-arginine + glycine Sus scrofa
-
 L-ornithine + guanidinoacetate
-
 r
2.1.4.1 L-ornithine + guanidinoacetate Sus scrofa
-
 L-arginine + glycine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.4.1 Homo sapiens
-
 human
-
2.1.4.1 Rattus norvegicus
-
 rat
-
2.1.4.1 Sus scrofa
-
 pig, porcine, hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.4.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.4.1 brain
-
 Homo sapiens
-
2.1.4.1 brain
-
 Rattus norvegicus
-
2.1.4.1 brain
-
 Sus scrofa
-
2.1.4.1 kidney
-
 Sus scrofa
-
2.1.4.1 liver
-
 Sus scrofa
-
2.1.4.1 muscle
-
 Sus scrofa
-
2.1.4.1 pancreas
-
 Sus scrofa
-
2.1.4.1 proximal tubule
-
 Homo sapiens
-
2.1.4.1 proximal tubule
-
 Rattus norvegicus
-
2.1.4.1 spleen
-
 Homo sapiens
-
2.1.4.1 spleen
-
 Rattus norvegicus
-
2.1.4.1 spleen
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.4.1 1.25
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.4.1 canavanine + ornithine
-
 Homo sapiens arginine + canaline
-
 r
2.1.4.1 canavanine + ornithine
-
 Rattus norvegicus arginine + canaline
-
 r
2.1.4.1 canavanine + ornithine
-
 Sus scrofa arginine + canaline
-
 r
2.1.4.1 L-arginine + glycine
-
 Homo sapiens L-ornithine + guanidinoacetate
-
 r
2.1.4.1 L-arginine + glycine
-
 Rattus norvegicus L-ornithine + guanidinoacetate
-
 r
2.1.4.1 L-arginine + glycine
-
 Sus scrofa L-ornithine + guanidinoacetate
-
 r
2.1.4.1 L-ornithine + guanidinoacetate
-
 Sus scrofa L-arginine + glycine
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.4.1 dimer
-
 Sus scrofa
2.1.4.1 dimer 2 * 44000, SDS-PAGE Homo sapiens
2.1.4.1 dimer 2 * 42000, alpha-transamidinase, SDS-PAGE, 2 * 44000, beta-transamidinase, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.4.1 7.5
-
-
 Sus scrofa