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Literature summary extracted from

  • Wargnies, B.; Lauwers, N.; Stalon, V.
    Structure and properties of the putrescine carbamoyltransferase of Streptococcus faecalis (1979), Eur. J. Biochem., 101, 143-12.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.6 phosphate
-
 Enterococcus faecalis
2.1.3.6 spermidine
-
 Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.6 0.029
-
 putrescine
-
 Enterococcus faecalis
2.1.3.6 0.35
-
 spermine
-
 Enterococcus faecalis
2.1.3.6 2.9
-
 1,3-diaminopropane
-
 Enterococcus faecalis
2.1.3.6 6.6
-
 1,6-diaminohexane
-
 Enterococcus faecalis
2.1.3.6 7.7
-
 cadaverine
-
 Enterococcus faecalis
2.1.3.6 13
-
 ornithine
-
 Enterococcus faecalis
2.1.3.6 19.7
-
 spermidine
-
 Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.6 40000
-
 3 * 40000, treatment with glutaraldehyde, SDS-PAGE Enterococcus faecalis
2.1.3.6 140000
-
 gel filtration Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.6 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.6
-
 Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.6 508.3
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.6 carbamoyl phosphate + 1,3-diaminopropane
-
 Enterococcus faecalis phosphate + N1-carbamoyl-1,3-diaminopropane
-
 ?
2.1.3.6 carbamoyl phosphate + 1,6-diaminohexane
-
 Enterococcus faecalis phosphate + N1-carbamoyl-1,6-diaminohexane
-
 ?
2.1.3.6 carbamoyl phosphate + cadaverine
-
 Enterococcus faecalis phosphate + N-carbamoylcadaverine
-
 ?
2.1.3.6 carbamoyl phosphate + spermidine
-
 Enterococcus faecalis phosphate + N-carbamoylspermidine
-
 ?
2.1.3.6 carbamoyl phosphate + spermine
-
 Enterococcus faecalis phosphate + N-carbamoylspermine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.6 trimer 3 * 40000, treatment with glutaraldehyde, SDS-PAGE Enterococcus faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.6 55
-
 carbamoylphosphate, diphosphate, ornithine, 2,4-diaminobutyrate and norvaline protect against denaturation Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.6 7 9
-
 Enterococcus faecalis
2.1.3.6 9
-
-
 Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.6 2.5
-
 spermidine vs. putrescine Enterococcus faecalis
2.1.3.6 4.5
-
 spermidine vs. carbamoylphosphate Enterococcus faecalis
2.1.3.6 6
-
 phosphate vs. carbamoylphosphate Enterococcus faecalis
2.1.3.6 8.5
-
 phosphate vs. putrescine Enterococcus faecalis
2.1.3.6 13.5
-
 phosphate vs. ornithine Enterococcus faecalis