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Literature summary extracted from

  • Bellocco, E.; Di Salvo, C.; Lagana, G.; Galtieri, A.; Ficarra, S.; Kotyk, A.; Leuzzi, U.
    Ornithine carbamoyltransferase from Spinacia oleracea: purification and characterization (2002), Biol. Plant., 45, 533-538.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 ornithine competitive vs. carbamoylphosphate in the presence of phosphate Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 40000
-
 3 * 40000, SDS-PAGE Spinacia oleracea
2.1.3.3 118000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 ammonium sulfate, heat, activated charcoal, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.3 carbamoyl phosphate + L-ornithine = phosphate + L-citrulline ordered bi-bi-sequential reaction mechanism, carbamoylphosphate binds first followed by L-ornithine, L-citrulline leaves first followed by phosphate Spinacia oleracea
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 436
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Spinacia oleracea phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 40000, SDS-PAGE Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.3 56
-
 half-live: 99 min Spinacia oleracea
2.1.3.3 58
-
 half-life: 60 min Spinacia oleracea
2.1.3.3 64
-
 half-life: 11 min Spinacia oleracea
2.1.3.3 68
-
 half-life: 10.5 min, half-life increases to 45 min in the presence of 50 mM ornithine Spinacia oleracea