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Literature summary extracted from

  • Lee, Y.; Choi, Y.A.; Hwang, I.D.; Kim, S.G.; Kwon, Y.M.
    cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding site (2001), Plant Mol. Biol., 46, 651-660.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 expression of isoform 1 and 2 in Escherichia coli Canavalia lineata

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.3 H121R isoform 2, 80% increase in ornithine dependent activity, 64% decrease in canaline-dependent activity Canavalia lineata
2.1.3.3 L118M isoform 2, 515% increase in ornithine dependent activity, 54% decrease in canaline-dependent activity Canavalia lineata
2.1.3.3 L118M isoform 2, 617% increase in ornithine dependent activity, 55% decrease in canaline-dependent activity Canavalia lineata

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Canavalia lineata
-
 2 isoforms
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 leaf
-
 Canavalia lineata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 0.0928
-
 isoenzyme 2 with ornithine Canavalia lineata
2.1.3.3 0.574
-
 isoenzyme 1 with canaline Canavalia lineata
2.1.3.3 1.24
-
 isoenzyme 2 with canaline Canavalia lineata
2.1.3.3 8.31
-
 isoenzyme 1 with ornithine Canavalia lineata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + canaline isoform 2 shows 13times higher canaline-dependent transcarbamoylase activity than ornithine-dependent transcarbamoylase activity Canavalia lineata O-ureidohomoserine + phosphate
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine isoform 1 shows 14times higher ornithine-dependent transcarbamoylase activity than canaline-dependent transcarbamoylase activity Canavalia lineata phosphate + L-citrulline
-
 ?