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Literature summary extracted from

  • Lee, Y.; Jun, B.O.; Kim, S.G.; Kwon, Y.M.
    Purification of ornithine carbamyltransferase from kidney bean (Phaseolus vulgaris) leaves and comparison of the properties of the enzyme from canavanine-containing and -deficient plants (1998), Planta, 205, 375-379.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 L-cysteine 10 mM, 90% inhibition, noncompetitive vs. ornithine Phaseolus vulgaris
2.1.3.3 S-carbamoyl cysteine 10 mM, 90% inhibition Phaseolus vulgaris
2.1.3.3 Tris 50 mM, more than 80% inhibition Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 36000
-
 3 * 36000, SDs-PAGE Phaseolus vulgaris
2.1.3.3 109000
-
-
 Canavalia lineata
2.1.3.3 109000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Brassica juncea
-
-
-
2.1.3.3 Canavalia brasiliensis
-
-
-
2.1.3.3 Canavalia gladiata
-
-
-
2.1.3.3 Canavalia lineata
-
-
-
2.1.3.3 Canavalia rosea
-
-
-
2.1.3.3 Glycine max
-
-
-
2.1.3.3 Lablab purpureus
-
-
-
2.1.3.3 Macropsychanthus megacarpus
-
-
-
2.1.3.3 Mus musculus
-
-
-
2.1.3.3 Oryctolagus cuniculus
-
-
-
2.1.3.3 Perilla frutescens
-
-
-
2.1.3.3 Phaseolus vulgaris
-
-
-
2.1.3.3 Pisum sativum
-
-
-
2.1.3.3 Solanum lycopersicum
-
-
-
2.1.3.3 Solanum melongena
-
-
-
2.1.3.3 Wisteria floribunda
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 delta-N-(phosphonoacetyl)-L-ornithine-Sepharose 6B affinity chromatography Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 leaf
-
 Phaseolus vulgaris
-
2.1.3.3 leaf
-
 Pisum sativum
-
2.1.3.3 leaf
-
 Glycine max
-
2.1.3.3 leaf
-
 Solanum lycopersicum
-
2.1.3.3 leaf
-
 Solanum melongena
-
2.1.3.3 leaf
-
 Brassica juncea
-
2.1.3.3 leaf
-
 Lablab purpureus
-
2.1.3.3 leaf
-
 Perilla frutescens
-
2.1.3.3 leaf
-
 Canavalia lineata
-
2.1.3.3 leaf
-
 Canavalia rosea
-
2.1.3.3 leaf
-
 Canavalia gladiata
-
2.1.3.3 leaf
-
 Canavalia brasiliensis
-
2.1.3.3 leaf
-
 Macropsychanthus megacarpus
-
2.1.3.3 leaf
-
 Wisteria floribunda
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 526
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Mus musculus phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Oryctolagus cuniculus phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Pisum sativum phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Glycine max phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Solanum lycopersicum phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Solanum melongena phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Brassica juncea phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Lablab purpureus phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Perilla frutescens phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Canavalia lineata phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Canavalia rosea phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Canavalia gladiata phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Canavalia brasiliensis phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Macropsychanthus megacarpus phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Wisteria floribunda phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine very low activity with canaline Phaseolus vulgaris phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer
-
 Pisum sativum
2.1.3.3 trimer
-
 Canavalia lineata
2.1.3.3 trimer 3 * 36000, SDs-PAGE Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 8
-
-
 Pisum sativum
2.1.3.3 8
-
-
 Canavalia lineata