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Literature summary extracted from

  • Sanchez, R.; Baetens, M.; Van De Casteele, M.; Roovers, M.; Legrain, C.; Glansdorff, N.
    Ornithine carbamoyltransferase from the extreme thermophile Thermus thermophilus. Analysis of the gene and characterization of the protein (1997), Eur. J. Biochem., 248, 466-474.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 expression in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 delta-N-(phosphonacetyl)-L-ornithine
-
 Thermus thermophilus
2.1.3.3 Delta-N-Phosphonoacetyl-L-ornithine
-
 Thermus thermophilus
2.1.3.3 phosphate
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 36000
-
 3 * 36000, SDs-PAGE Thermus thermophilus
2.1.3.3 110000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 recombinant enzyme, thermodenaturation at 85°C, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 3.33
-
 recombinant enzyme Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Thermus thermophilus phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 36000, SDs-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.3.3 65
-
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.3 85
-
 half-life: 18 min, 10 mM ornithine and phosphate increase half-life 5fold and 7fold respectively Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 8
-
 at an ornithine concentration around the Km value, at higher concetration the pH optimum shifts to pH 7.0 Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.3 0.00001
-
 delta-N-(phosphonacetyl)-L-ornithine
-
 Thermus thermophilus