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Literature summary extracted from

  • Kalousek, F.; Francois, B.; Rosenberg, L.E.
    Isolation and characterization of ornithine transcarbamylase from normal human liver (1978), J. Biol. Chem., 253, 3939-3944.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.3.3 cetyltrimethylammonium stabilizes enzyme in crude extract against heat inactivation in presence of the detergent Homo sapiens
2.1.3.3 loss of activity after the second thawing Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 114000
-
 gel filtration, glycerol density gradient centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 233
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 -20°C, 50% glycerol, 10 mM potassium phosphate, pH 7.0, 1 mM 2-mercaptoethanol, 4 months, no loss of activity Homo sapiens
2.1.3.3 -50°C, indefinitely Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Homo sapiens phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer SDS-PAGE under reducing conditions Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.3 -50 60 stable Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 7.7
-
 triethanolamine buffer, 85% of maximal activity at pH 8.5, 25% of maximal activity at pH 6.8 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.3.3 6.8 8.5 approx. 85% of maximal activity at pH 6.8, approx. 25% of maximal activity at pH 8.5 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.3.3 5.8 8.2 120 min, 37°C, stable Homo sapiens