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Literature summary extracted from

  • Lusty, C.J.; Jilka, R.L.; Nietsch, E.H.
    Ornithine transcarbamylase of rat liver. Kinetic, physical, and chemical properties (1979), J. Biol. Chem., 254, 10030-10036.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 glutamine
-
 Rattus norvegicus
2.1.3.3 histidine
-
 Rattus norvegicus
2.1.3.3 L-2-aminobutyrate
-
 Rattus norvegicus
2.1.3.3 L-isoleucine
-
 Rattus norvegicus
2.1.3.3 L-leucine
-
 Rattus norvegicus
2.1.3.3 L-lysine weak Rattus norvegicus
2.1.3.3 L-norvaline
-
 Rattus norvegicus
2.1.3.3 L-valine
-
 Rattus norvegicus
2.1.3.3 methionine
-
 Rattus norvegicus
2.1.3.3 additional information not inhibited by arginine Rattus norvegicus
2.1.3.3 phosphate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 39600
-
 3 * 39600, SDS-PAGE, sedimentation equilibrium analysis in 6 M guanidine-HCl Rattus norvegicus
2.1.3.3 39900
-
 3 * 39900, amino acid sequence Rattus norvegicus
2.1.3.3 112000
-
 sedimentation equilibrium analysis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 885
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 -70°C, 2% loss of activity per month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Rattus norvegicus phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + lysine 4.3% of the rate with ornithine Rattus norvegicus phosphate + homocitrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 39600, SDS-PAGE, sedimentation equilibrium analysis in 6 M guanidine-HCl Rattus norvegicus
2.1.3.3 trimer 3 * 39900, amino acid sequence Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 7.6
-
 triethanolamine/acetate buffer Rattus norvegicus
2.1.3.3 7.9
-
 HEPES/NaOH buffer Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.3.3 6.7 8.5 approx. 50% of maximal activity at pH 6.7 and 8.5, triethanolamine/acetate buffer Rattus norvegicus
2.1.3.3 7.2 8.3 71% of maximal activity at pH 7.2, 94% of maximal activity at pH 8.3, HEPES/NaOH buffer Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.3.3 6.8 9 60 min, stable Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.3 0.071
-
 L-norvaline
-
 Rattus norvegicus
2.1.3.3 0.25
-
 phosphate
-
 Rattus norvegicus
2.1.3.3 0.58
-
 L-alpha-aminobutyrate
-
 Rattus norvegicus
2.1.3.3 1.2
-
 L-leucine
-
 Rattus norvegicus
2.1.3.3 10.5
-
 L-isoleucine
-
 Rattus norvegicus
2.1.3.3 12.5
-
 L-valine
-
 Rattus norvegicus
2.1.3.3 40
-
 L-histidin
-
 Rattus norvegicus