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Literature summary extracted from

  • Xiong, X.; Anderson, P.M.
    Purification and properties of ornithine carbamoyl transferase from liver of Squalus acanthias (1989), Arch. Biochem. Biophys., 270, 198-207.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 citrulline competitive to carbamoyl phosphate Squalus acanthias
2.1.3.3 L-norvaline
-
Squalus acanthias
2.1.3.3 MgCl2 10 mM, 14% inhibition Squalus acanthias
2.1.3.3 NaCl 50 mM, 25% inhibition Squalus acanthias
2.1.3.3 ornithine
-
Squalus acanthias
2.1.3.3 phosphate competitive vs. carbamoylphosphate Squalus acanthias

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.3.3 mitochondrion
-
Squalus acanthias 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 38000
-
3 * 38000, SDS-PAGE Squalus acanthias
2.1.3.3 113000
-
gel filtration Squalus acanthias

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine Squalus acanthias catalyzes the last step of mitochondrial citrulline synthesis phosphate + L-citrulline
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Squalus acanthias
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 DEAE-Sephadex, hydoxyapatite C, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography Squalus acanthias

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.3 carbamoyl phosphate + L-ornithine = phosphate + L-citrulline mechanism Squalus acanthias

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 liver
-
Squalus acanthias
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 27.1
-
-
Squalus acanthias

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 -20°C, 0.05 potassium phosphate buffer, pH 7.6, 1 month, no loss of activity Squalus acanthias

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
Squalus acanthias phosphate + L-citrulline
-
?
2.1.3.3 carbamoyl phosphate + L-ornithine catalyzes the last step of mitochondrial citrulline synthesis Squalus acanthias phosphate + L-citrulline
-
?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 38000, SDS-PAGE Squalus acanthias

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.3 0.06
-
ornithine
-
Squalus acanthias