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Literature summary extracted from

  • Bates, M.; Weiss, R.L.; Clarke, S.
    Ornithine transcarbamylase from Neurospora crassa: purification and properties (1985), Arch. Biochem. Biophys., 239, 172-183.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.3.3 glycerol, 20% v/v, stabilizes during freeze-thawing Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 37000
-
 3 * 37000, SDS-PAGE Neurospora crassa
2.1.3.3 110000
-
 SDS-PAGE of dimethyl suberimidate cross-linked enzyme Neurospora crassa
2.1.3.3 115000
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine Neurospora crassa enzyme is involved in the biosynthesis of arginine in many organisms and participates in the urea cycle of mammals phosphate + L-citrulline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 ammonium sulfate, 70°C for 25 min, gel filtration, ion-exchange chromatography Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 251
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Neurospora crassa phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine enzyme is involved in the biosynthesis of arginine in many organisms and participates in the urea cycle of mammals Neurospora crassa phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 37000, SDS-PAGE Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 9.5
-
-
 Neurospora crassa